Circulating Cell-Free Nucleic Acids：Characteristics，Purification，Applications；Quality Control Analysis。Rapidly turned over,half-life of 30mins-2hours，Mutation percentage increases as cancer advances。From Blood to Biomarkers。Blood collection tubes。Stabilizer to prevent White blood cell lysis Stable at rt for 2 weeks。Take Home Message：cfDNA is dilute,highly fragmented and sensitive to storage time and temperature；Cancer biomarkers can be detected in cfDNA；The effectiveness of liquid biopsies has been demonstrated。
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