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首页> 外文会议>The 3rd International Conference on Bioinformatics and Biomedical Engineering(iCBBE 2009)(第三届生物信息与生物医学工程国际会议）论文集 >Preliminary Production and Characterization of Anti- Monocrotophos Monoclonal Antibody
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Preliminary Production and Characterization of Anti- Monocrotophos Monoclonal Antibody

机译：抗久效磷单克隆抗体的初步制备与鉴定

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Monocrotophos as the hapten was coupled the carrier proteins ovalbumin (OVA) and bovine serum albumin (BSA) by Glutaral Method to synthesis artificial antigen and coating antigen. The identifications of synthetic antigens were by ultraviolet absorption spectrum scan and SDS-PAGE methods. And then BALB/C mices were immunized with immunogen (hapten-OVA). The result showed that UV spectrogram of synthetic antigens was changed when compared with OVA and BSA, electrophoretic mobility between synthetic antigens and carrier proteins was different. The coupling molar ratios of the hapten and carrier proteins were 23.3:1 for hapten-OVA, 13.7:1 for hapten-BSA. Through examinations, the titer of the antiserum of mice is 1:409600.It could be concluded that Monocrotophos artificial antigen was synthesized and mice were immunized successfully. After cell fusion and cloned strain was established, the characterization of antibody was identified, the results indicated: the subclass was IgM, the molecular weight was 100.6KDa, and the chromosomal numbers were between 97 and 104. With large-scale preparation of this monoclonal antibody, it was purified and analyzed by SDS-PAGE. That provided a basis for further establishing immunological assay detection and for Monocrotophos residue and related performances on pesticide studies.
机译：久效磷作为半抗原，通过戊二醛法将载体蛋白卵清蛋白（OVA）和牛血清白蛋白（BSA）偶联在一起，合成人工抗原和包被抗原。通过紫外吸收光谱扫描和SDS-PAGE方法鉴定合成抗原。然后用免疫原（半抗原-OVA）免疫BALB / C小鼠。结果表明，与OVA和BSA相比，合成抗原的紫外光谱图发生了变化，合成抗原与载体蛋白之间的电泳迁移率不同。半抗原-载体的半抗原和载体蛋白的偶联摩尔比为23.3：1，半抗原-BSA的偶联摩尔比为13.7：1。经检测，小鼠抗血清效价为1：409600。可以得出结论：久效磷人工抗原合成成功，免疫小鼠。建立细胞融合克隆株后，鉴定抗体的特征，结果表明：亚类为IgM，分子量为100.6KDa，染色体数目为97-104。抗体，将其纯化并通过SDS-PAGE分析。这为进一步建立免疫学检测方法和久效磷残留及农药研究相关性能提供了基础。

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