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首页> 外文会议>The 3rd International Conference on Bioinformatics and Biomedical Engineering(iCBBE 2009)(第三届生物信息与生物医学工程国际会议）论文集 >Cloning, expression analysis and sequence characterization of a monoterpene synthase gene, PaLis, from apricot (Prunus armeniaca)
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Cloning, expression analysis and sequence characterization of a monoterpene synthase gene, PaLis, from apricot (Prunus armeniaca)

机译：杏（Prunus armeniaca）单萜合酶基因PaLis的克隆，表达分析和序列表征

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摘要

Fruit fragrance compounds include more than a dozen chemical classes, such as organic acids, alcohols and terpenes etc. In this study, we cloned the full length cDNA encoding a linalool synthase, one of the monoterpene synthases from ‘Jintaiyang' apricot (Prunus armeniaca), using RACE (Rapid Amplification of cDNA Ends) strategy. The linalool synthase gene was named PaLis, and it was expressed in fruit and flower revealed by RTPCR. The full length PaLis cDNA contained a complete open reading frame (ORF) of 1815 bp encoding 605 amino acids. The PaLis gene showed two typical conserved motives of terpene synthases, i.e DDXXD and (N,D)D(L,I,V)X(S, t)XXXE, which are involved in the coordination of divalent cations. It also displayed the N-terminal peptide sequence RR(X)8W which is essential for the enzymatic activity of many monoterpene synthases. Another typical motif frequently found in such enzymes is LNLYEASYHS. The molecular weight (Mw) and isoeletric point (pI) of PaLis were predicted to be 70.6 kDa and 5.34, respectively. Amino acid comparison showed that the PaLis shared 36.9% to 68.2% similarities to other monoterpene synthase genes. The PaLis sequences cloned herein provide a molecular basis for the synthesis of monoterpenoids in apricot fruit, which is useful for elevating the level of fragrance compounds and thereby improving the quality of the production.
机译：水果香料化合物包括十多种化学类别，例如有机酸，醇和萜烯等。在这项研究中，我们克隆了编码芳樟醇合酶的全长cDNA，芳樟醇合酶是“金泰阳”杏（李子）的单萜合酶之一。 ，使用RACE（cDNA末端快速扩增）策略。芳樟醇合成酶基因命名为PaLis，通过RTPCR揭示在水果和花朵中表达。全长PaLis cDNA包含1815 bp的完整开放阅读框（ORF），编码605个氨基酸。 PaLis基因显示了萜烯合酶的两个典型保守动机，即DDXXD和（N，D）D（L，I，V）X（S，t）XXXE，它们参与二价阳离子的配位。它还显示了N端肽序列RR（X）8W，这对许多单萜合酶的酶促活性至关重要。在此类酶中经常发现的另一个典型基序是LNLYEASYHS。 PaLis的分子量（Mw）和等电点（pI）分别预测为70.6 kDa和5.34。氨基酸比较显示，PaLis与其他单萜合酶基因具有36.9％至68.2％的相似性。本文克隆的PaLis序列为杏果实中单萜类化合物的合成提供了分子基础，可用于提高芳香化合物的含量，从而提高生产质量。

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