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首页> 外文会议>12th Symposium on Bioluminescence and Chemiluminescence, Apr 5-9, 2002, Robinson College, University of Cambridge, UK >RED SHIFTS IN FIREFLY BIOLUMINESCENCE SPECTRA
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RED SHIFTS IN FIREFLY BIOLUMINESCENCE SPECTRA

机译：全生物发光光谱中的红色碎片

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摘要

The firefly bioluminescence is currently of the great interest for researchers as an example of effective bioconversion of energy from a chemical reaction into light. Moreover, the firefly bioluminescent reaction is widely used as a very sensitive bioassay for ATP detection. Nevertheless, the mechanism of light-emitting species formation in firefly luciferase reactions has not been entirely understood yet. There is no unanimous opinion on the origin of colour differences in firefly bioluminescence. At least three alternative mechanisms of luciferin-luciferase interactions responsible for characteristics of luminescence have been under discussion: (1) enzyme provides basic vicinity for oxyluciferin, assisting its tautomerisation; (2) the polarity of the active site influences on the electron-excited states of oxyluciferin; (3) conformation of active site determines the twisted or flat structure of oxyluciferin molecule. In this work we tried to affect with different factors on the emission spectra of the reaction catalysed by L. mingrelica luciferase. The temperature of the reaction mixture and concentration of fluorescent molecules (luciferin, oxyluciferin) and ethanol were varied at pH 7.8.
机译：萤火虫的生物发光目前正成为研究人员的极大兴趣，以作为能量从化学反应到光的有效生物转化的一个例子。此外，萤火虫生物发光反应被广泛用作ATP检测的非常灵敏的生物检测方法。然而，萤火虫荧光素酶反应中发光物质形成的机理尚未完全了解。萤火虫生物发光中的颜色差异的起源尚无一致意见。萤光素与萤光素酶相互作用引起发光特性的至少三种替代机制已在讨论中：（1）酶为氧化萤光素提供了基本的邻近区域，有助于其互变异构化； （2）活性位点的极性影响氧化荧光素的电子激发态； （3）活性位点的构象决定了氧化荧光素分子的扭曲或扁平结构。在这项工作中，我们尝试以不同的因素影响由明格里克荧光素酶催化的反应的发射光谱。反应混合物的温度以及荧光分子（荧光素，氧化荧光素）和乙醇的浓度在pH 7.8下变化。

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