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首页> 外文会议>12th Symposium on Bioluminescence and Chemiluminescence, Apr 5-9, 2002, Robinson College, University of Cambridge, UK >RELATIONSHIP BETWEEN SPECTRAL DISTRIBUTION OF VIBRIO FISCHERI STRAIN Yl BIOLUMINESCENCE AND INTRACELLULAR LEVEL OF ITS FLUORESCENT PROTEINS
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RELATIONSHIP BETWEEN SPECTRAL DISTRIBUTION OF VIBRIO FISCHERI STRAIN Yl BIOLUMINESCENCE AND INTRACELLULAR LEVEL OF ITS FLUORESCENT PROTEINS

机译：费氏弧菌菌株Yl的生物发光光谱分布与其荧光蛋白的细胞内水平之间的关系

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摘要

Yellow emitting luminous bacterium Vibrio fischeri strain Y1 produces diverse fluorescent proteins. In particular, yellow fluorescent protein (YFP) is of functional importance to induce the yellow bioluminescence with a maximum around 540 nm in the luciferase reaction. YFP exists as the two active forms, both of which are capable of being the secondary emitter. Besides YFP, V. fischeri Y1 has a blue fluorescent protein (Yl-BFP), whose cellular level may be comparable to the YFP level, but its function is unknown. It has been reported that V. fischeri Y1 alters the bioluminescence color during the cell growth, depending on the cellular level of YFP. For the V. fischeri Y1 bioluminescence, the intensity ratio of yellow emission to blue one (Y/B ratio) increases with increasing cell density late in the logarithmic phase. The cellular levels of luciferase and YFP are suggested to be closely related to cell density during growth. Based on the kinetic analysis of the in vitro luciferase reaction, it has been suggested that the interaction between the luciferase peroxyhemiacetal intermediate, leading to the formation of the primary emitter, and YFP may be responsible for the production of the electronically excited YFP (YFP~*). In this study, the spectral distribution of the V. fischeri Y1 bioluminescence during the cell growth at different temperatures has been characterized focussing attentions on Y/B ratio and fluorescence emission from the protein extracts.
机译：黄色发光发光细菌费氏弧菌Y1产生多种荧光蛋白。尤其是，黄色荧光蛋白（YFP）在诱导黄色生物发光方面具有重要的功能，荧光素酶反应中的最大荧光波长约为540 nm。 YFP以两种活动形式存在，两者都可以作为辅助发射器。除YFP外，费氏弧菌Y1还具有蓝色荧光蛋白（Y1-BFP），其细胞水平可能与YFP水平相当，但其功能尚不清楚。据报道，费氏弧菌Y1在细胞生长期间改变生物发光颜色，这取决于YFP的细胞水平。对于费氏弧菌Y1生物发光，黄色发射与蓝色发射的强度比（Y / B比）在对数期后期随着细胞密度的增加而增加。荧光素酶和YFP的细胞水平建议与生长过程中的细胞密度密切相关。根据体外萤光素酶反应的动力学分析，已表明萤光素酶过氧半缩醛中间体与导致初级发射极形成的相互作用与YFP可能是电子激发YFP（YFP〜 *）。在这项研究中，伏氏弧菌Y1生物发光在不同温度下细胞生长过程中的光谱分布已被表征为重点关注蛋白质提取物的Y / B比和荧光发射。

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