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首页> 外文会议>12th Symposium on Bioluminescence and Chemiluminescence, Apr 5-9, 2002, Robinson College, University of Cambridge, UK >B1OLUMINESCENT ASSAY OF TOTAL BACTERIAL CONTAMINATION (TBC) IN FOOD SAMPLES AND DRINKING WATER USING FILTRAVETTE~(TM)
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B1OLUMINESCENT ASSAY OF TOTAL BACTERIAL CONTAMINATION (TBC) IN FOOD SAMPLES AND DRINKING WATER USING FILTRAVETTE~(TM)

机译：使用FILTRAVETTE〜（TM）对食品样品和饮用水中的总细菌污染（TBC）进行B1发光分析

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摘要

The bioluminescent assay of TBC is one of the most rapid, simple and economically reasonable methods among rapid microbiology methods developed for assessment of hygiene quality of food samples and drinking water. Most samples analyzed contain excess of non-bacterial ATP (sum of somatic and free ATP) and/or a low number of bacteria, and therefore a special pretreatment of the sample is required and this is laborious and time consuming. To overcome this obstacle we used special luminometric polystyrene microcuvettes (h 13 mm, φ 10 mm), Filtravette~(TM), with the bottom made of bacterial membrane filter (pore size 0.45 μm). The design of Filtravette~(TM) allows one to concentrate bacteria cells, extract bacterial ATP and measure bioluminescent signal in the same cuvette. This simplifies the analysis, enhances its accuracy and decreases detection limit of bacteria cells. The combined application of Filtravette~(TM) and highly sensitive ATP-reagent developed in our laboratory permitted us to detect as low as ～ 200 bacteria per Filtravette~(TM).
机译：TBC的生物发光分析是为评估食品和饮用水的卫生质量而开发的“快速微生物学”方法中最快速，简单且经济上合理的方法之一。分析的大多数样品都含有过量的非细菌ATP（体细胞和游离ATP的总和）和/或细菌数量少，因此需要对样品进行特殊的预处理，这既费力又费时。为了克服这一障碍，我们使用了特殊的发光聚苯乙烯微孔（h 13 mm，φ10 mm），FiltravetteTM，底部由细菌膜过滤器制成（孔径为0.45μm）。 FiltravetteTM的设计使人们可以在同一比色皿中浓缩细菌细胞，提取细菌ATP并测量生物发光信号。这简化了分析，提高了分析的准确性，并降低了细菌细胞的检出限。 Filtravette〜（TM）和我们实验室开发的高灵敏度ATP试剂的组合应用使我们能够检测到每个Filtravette〜（TM）约200个细菌。

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