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首页> 外文会议>12th Symposium on Bioluminescence and Chemiluminescence, Apr 5-9, 2002, Robinson College, University of Cambridge, UK >CHEMILUMINESCENCE OF ISOLATED HUMAN LEUKOCYTES INDUCED BY STREPTOCOCCUS MUTANS IS GENERATED EXTRACELLULARLY IN THE ABSENCE OF PHAGOCYTOSIS
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CHEMILUMINESCENCE OF ISOLATED HUMAN LEUKOCYTES INDUCED BY STREPTOCOCCUS MUTANS IS GENERATED EXTRACELLULARLY IN THE ABSENCE OF PHAGOCYTOSIS

机译：在缺乏吞噬作用的情况下，由链球菌引起的孤立人类白细胞的化学发光在细胞外产生

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The production of reactive oxygen species (ROS) by phagocytic cells is one of the basic microbicidal mechanisms. Microbial invaders are phagocytosed and destroyed by ROS inside cells. However, ROS are also generated extracellularly (before a phagocytic vesicle is fully enclosed or during frustrated phagocytosis) or they can leak out of cells. Extracellular ROS induce a destruction of the surrounding cells and tissues. Thus, there is an effort to eliminate extracellular ROS and their effects pharmacologically, without disturbing the microbicidal functions of phagocytes. When compared to other methods used (spectrophotometry and flow cytometry) luminometric methods enable continual analysis of the respiratory burst of phagocytes. In this case, cells are incubated with a selected activator and a tested substance directly in the measuring chamber of a luminometer. Such a continual analysis reflects the total ROS production and could be employed to distinguish between its intra- and extracellular components. Two approaches are used for this purpose: Ⅰ) using luminophores with different permeability through the cell membrane, Ⅱ) using antioxidant enzymes such as superoxide dismutase and catalase. However, a certain amount of substances present in the cell environs can be ingested when the phagocytic vesicles are enclosed. Therefore, these approaches are not fully selective. The aim of this study was to detect differences between the chemiluminescence (CL) responses of phagocytes triggered by bacteria in the absence and presence of phagocytosis.
机译：吞噬细胞产生活性氧（ROS）是基本的杀微生物机制之一。微生物入侵者被细胞内的ROS吞噬并破坏。但是，ROS还是在细胞外产生的（在吞噬泡完全被封闭之前或在吞噬过程受阻期间），或者它们可以从细胞中漏出。细胞外ROS诱导周围细胞和组织的破坏。因此，在不干扰吞噬细胞的杀微生物功能的情况下，努力在药理上消除细胞外ROS及其作用。与其他使用的方法（分光光度法和流式细胞仪）相比，发光法可对吞噬细胞的呼吸爆发进行连续分析。在这种情况下，将细胞与选定的激活剂和被测物质直接在光度计的测量室中孵育。这样的连续分析反映了总ROS的产生，可用于区分其细胞内和细胞外成分。为此目的，使用了两种方法：Ⅰ）使用具有不同渗透性的发光体穿过细胞膜，Ⅱ）使用抗氧化酶，如超氧化物歧化酶和过氧化氢酶。但是，当吞噬吞噬囊泡被封闭时，细胞环境中存在的一定量的物质可以被摄入。因此，这些方法不是完全选择性的。这项研究的目的是检测在不存在和存在吞噬作用的情况下由细菌触发的吞噬细胞的化学发光（CL）反应之间的差异。

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