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首页> 外文会议>13th Annual Conference of the Chinese Society of Micro-Nano Technology >A Portable Instrument Integrated with Temperature Control Equipment for Field Rapid Test of Bacteria Based on Bioluminescent Magnetic Immunoassay
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A Portable Instrument Integrated with Temperature Control Equipment for Field Rapid Test of Bacteria Based on Bioluminescent Magnetic Immunoassay

机译：结合温度控制设备的便携式仪器，用于基于生物发光磁免疫测定的细菌现场快速检测

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摘要

A portable instrument was designed based on bioluminescent magnetic immunoassay. The instrument consisted of photon counting unit, central processing unit, temperature control unit and power supply unit. The temperature control equipment integrated into the instrument supplied a most suitable environment for incubation at 37 ℃, which would be more convenient for field test than large-scale heating apparatus used in laboratory. In this study, an improved simplified coupling method, in which immunomagnetic microbead coupled with biotinylated rabbit-anti-Escherichia coli antibody could capture Escherichia coli directly, took one step instead of the previous method of two steps. The Escherichia coli detecting result showed a good relationship in log-log domain between the intensity of luminescence and the concentration of Escherichia coli in the range from 23.00 CFU/mL to 2.25×107 CFU/mL with a correlation coefficient of 0.9657. The sensitivity of the detecting system was 0.4787. In the reproducibility test, the coefficient of variation was 4.48%. The instrument was portable, easy to use, low-cost, integrated with temperature control equipment and fast-testing, which would provide potential application in food hygiene and safety, environmental monitoring and disease diagnosis.
机译：基于生物发光磁免疫测定法设计了便携式仪器。该仪器由光子计数单元，中央处理器，温度控制单元和电源单元组成。仪器中集成的温度控制设备提供了最适合37℃温育的环境，比实验室使用的大型加热设备更便于现场测试。在这项研究中，一种改进的简化的偶联方法只用了一个步骤，而不是先前的两步方法，其中免疫磁微珠与生物素化的兔抗大肠杆菌抗体可以直接捕获大肠杆菌。大肠杆菌的检测结果在发光强度与大肠杆菌浓度在23.00 CFU / mL至2.25×107 CFU / mL的范围内，在log-log域中显示出良好的相关性，相关系数为0.9657。检测系统的灵敏度为0.4787。在再现性测试中，变异系数为4.48％。该仪器是便携式，易于使用，低成本，与温度控制设备集成在一起并进行快速测试的仪器，可在食品卫生和安全，环境监测和疾病诊断中提供潜在的应用。

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