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首页> 外文会议>13th International Symposium on Ceramics in Medicine and the Annual Meeting of the International Society for Ceramics in Medicine 22-26 November 2000 Bologna, Italy >Effect of Apatite Cements on Human Osteoblasts in Vitro
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Effect of Apatite Cements on Human Osteoblasts in Vitro

机译：磷灰石水泥对人成骨细胞的影响

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A conventional apatite cement (c-Ac) used for reconstruction of bony defect takes 30 min to set and decays upon exposure to liquid before setting. Therefore, we have developed two types of new ACs; fast-setting AC and anti-washout type AC. The aims of this study are to investigate the effect of ACs on cultured osteoblasts and to compare effects of new ACs on osteoblasts with that of c-AC. Disc shape set ACs with almost same surface roughness were prepared and evaluated by powder X-ray diffraction (XRD). The clonal human osteoblasts were cultured one each specimens. Plastic dish and sintered hydroxyapatite (HAP) were used as control. ACs showed the same apatitic XRD patterns with poor crystallinity when compared with sintered HAP. There were no differences in proliferation of osteoblasts among ACs and sintered HAP. The alkaline phosphatase activity and protein levels for type I collagen and osteocalcin of ACs were greater than those of sintered HAP. There was no difference among ACs. In conclusion, it is suggested that ACs could promote the differentiation of osteoblasts as compared with sintered HAP and plastic. Also, it is suggested that two types of new ACs would show the same cell response as c-AC in vivo
机译：用于重建骨缺损的常规磷灰石水泥（c-Ac）需花费30分钟的固化时间，并且在固化之前暴露于液体中会腐烂。因此，我们开发了两种类型的新型AC。快速设置交流电和防冲洗交流电。这项研究的目的是调查ACs对培养的成骨细胞的作用，并比较新型ACs对c-AC的作用对成骨细胞的作用。制备了具有几乎相同的表面粗糙度的圆盘形状的AC，并通过粉末X射线衍射（XRD）进行了评估。每个标本培养一个克隆的人类成骨细胞。将塑料皿和烧结的羟基磷灰石（HAP）用作对照。与烧结的HAP相比，AC表现出相同的磷化XRD模式，但结晶度较差。 AC和烧结的HAP之间成骨细胞的增殖没有差异。 ACs的I型胶原和骨钙素的碱性磷酸酶活性和蛋白水平均高于烧结HAP。 AC之间没有差异。结论表明，与烧结的HAP和塑料相比，AC可以促进成骨细胞的分化。另外，建议两种新型AC在体内显示出与c-AC相同的细胞反应

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