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首页> 外文会议>13th International Symposium on Ceramics in Medicine and the Annual Meeting of the International Society for Ceramics in Medicine 22-26 November 2000 Bologna, Italy >In Vitro Carbonated Apatite Precipitation on Biphasic Calcium Phosphate Pellets Presenting Various HA/beta-TCP Ratios
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In Vitro Carbonated Apatite Precipitation on Biphasic Calcium Phosphate Pellets Presenting Various HA/beta-TCP Ratios

机译：呈现各种HA /β-TCP比值的双相磷酸钙小球上的体外碳酸化磷灰石沉淀

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摘要

To study the influence of calcium phosphate ceramic composition on biological apatite formation, pellets made of hydroxyapatite (HA), beta-tricalcium phosphate (beta-TCP) and two types of biphasic calcium phosphates (BCP) with HA/beta-TCP ratios of 25/75 and 75/25 were incubated in cell medium for 48 hours. Then pellets were removed from wells and surfaces were analyzed in Scanning Electron Microscopy equipped with an image analysis system and in Transmission Electron Microscopy (TEM) equipped with Electron microprobe analysis (EDX). pH and calcium concentrations were also measured in incubation wells. Newly formed carbonated apatite crystals were observed at the surface of all the pellets. However: 1. the amount of precipitated crystals was twice lower for pure beta-TCP 2 the apatite formation occured faster for biphasis pellets (HA/beta-TCP mixtures) Knowing that the biological apatite layer is involved in cells adhesion, these results may explain partially in vivo performances of biphasis calcium phosphate ceramics as bone substitutes.
机译：为了研究磷酸钙陶瓷成分对生物磷灰石形成的影响，使用羟基磷灰石（HA），β-磷酸三钙（β-TCP）和两种HA /β-TCP比率为25的两种双相磷酸钙（BCP）制成的颗粒/ 75和75/25在细胞培养基中孵育48小时。然后从孔中除去沉淀，并在配备有图像分析系统的扫描电子显微镜和配备有电子微探针分析（EDX）的透射电子显微镜（TEM）中对表面进行分析。在培养孔中也测量pH和钙浓度。在所有粒料的表面观察到新形成的碳酸磷灰石晶体。但是：1.纯β-TCP的沉淀晶体数量要少两倍。2双相微丸（HA /β-TCP混合物）的磷灰石形成速度更快。这些结果可能解释了生物磷灰石层参与细胞黏附，双相磷酸钙陶瓷作为骨替代品的部分体内性能。

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