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首页> 外文会议>13th International Symposium on Ceramics in Medicine and the Annual Meeting of the International Society for Ceramics in Medicine 22-26 November 2000 Bologna, Italy >In Vitro Interactions of Bone Marrow Cells with Carbonate and Fluoride Containing Apatites
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In Vitro Interactions of Bone Marrow Cells with Carbonate and Fluoride Containing Apatites

机译：含碳酸盐和氟化物的磷灰石在骨髓细胞中的体外相互作用

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The purpose of this study was to investigate the in vitro interactions between osteoclastlike cells and synthetic apatites. Carbonate-free (HAP), carbonate substituted (CHA), and carbonate- and fluoride-substituted (CFA) apatites were prepared by precipitation method and characterized using X-ray diffraction, infrared spectroscopy, scanning electron microscopy with energy dispersive system and thermo gravimetric analyses. Cells from the bone marrow of one-day old rabbit were seeded on the apatite substrates. After three days in cell culture, all the apatite substrates showed TRAP positive reaction. The intensity of the reaction of TRAP positive cells decreased in the order: CHA>HAP>CFA. SEM observation showed that the morphology of cells on the CFA and/or HAP discs resembled the phagocytes such as monocytes, rather than the osteoclast. These results indicate that carbonate ions in the apatite lattice encourage the cell activity while the fluoride ions inhibit phagocyte cell activity.
机译：本研究的目的是研究破骨细胞样细胞与合成磷灰石之间的体外相互作用。采用沉淀法制备无碳酸盐（HAP），碳酸盐取代（CHA）以及碳酸盐取代和氟取代（CFA）的磷灰石，并利用X射线衍射，红外光谱，带能量分散系统的扫描电子显微镜和热重分析法进行表征分析。将一天大兔子的骨髓细胞接种到磷灰石基质上。细胞培养三天后，所有磷灰石底物均显示出TRAP阳性反应。 TRAP阳性细胞的反应强度依次为：CHA> HAP> CFA。 SEM观察表明，CFA和/或HAP椎间盘上的细胞形态类似于单核细胞等吞噬细胞，而不是破骨细胞。这些结果表明磷灰石晶格中的碳酸根离子促进细胞活性，而氟离子抑制吞噬细胞活性。

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