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首页> 外文会议>18th International Symposium on Fine Chemistry and Functional Polymers,4th IUPAC International Symposium on Novel Materials and Their Synthesis(第18届国际精细化学和功能高分子学术讨论会暨第四届IUPAC 国际新型材料及其制备学术讨论会） >Formation of Biomimetic Membranes on Carboxylic Acid Terminated Self-assembled Monolayers For Metrology and Biosensor Applications
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Formation of Biomimetic Membranes on Carboxylic Acid Terminated Self-assembled Monolayers For Metrology and Biosensor Applications

机译：在计量学和生物传感器应用中，在端接有羧酸的自组装单分子膜上形成仿生膜

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1.Introduction Biomimetic membranes are synthetic phospholipid mixtures that may mimic the composition of various cell membranes, and are therefore frequently employed to enhance biocompatibility of implants and intracorporeous devices, create a pseudo-physiological environment for enzyme-based biosensors and bioreactors, and to study biological membrane processes.Activity and gating of ion channels, antimicrobial action of membrane lysing peptides, electrochemistry of biological charge transfer can all be studied in a controlled environment when using biomimetic membrane platforms. Moreover, analytical tools such as surface plasmon resonance (SPR) or quartz crystal microbalance (QCM) use biomimetic membrane modified sensor surfaces or chips, and artificial cell designs also rely extensively on these structures. It is therefore crucial to establish protocols to create biomimetic membranes in a reproducible way with well defined and controlled properties. Liposome deposition, where vesicles of phospholipid bilayers deliver the membrane to arbitrary substrates, is frequently used. This method is, however, sensitive to variations of the size and composition of the liposomes, and the physicochemical properties of the surface. While a unifying theory of liposome adhesion, rupture, collapse and concomitant fuse into bilayer membranes is yet to be introduced, we present an empirical study that follows the process of membrane formation from the liposome precursor through the deposition process to the structural characterization of the resulting membranes, for representative mammalian (DMPC) and bacteria -like (DMPC:DMPG 4:1) membranes, on a carboxylic-acid terminated self assembled monolayer, which can be easily created on various substrates and thus can be used to make membrane deposition substrate independent.
机译：1简介仿生膜是合成的磷脂混合物，可以模拟各种细胞膜的组成，因此常用于增强植入物和体内设备的生物相容性，为基于酶的生物传感器和生物反应器创造假生理环境，并进行研究当使用仿生膜平台时，可在受控环境中研究离子通道的活性和门控，膜裂解肽的抗菌作用，生物电荷转移的电化学。此外，诸如表面等离振子共振（SPR）或石英晶体微量天平（QCM）之类的分析工具使用仿生膜修饰的传感器表面或芯片，人工细胞设计也广泛依赖于这些结构。因此，至关重要的是要建立规程，以可再现的方式创建具有良好定义和控制的特性的仿生膜。经常使用脂质体沉积，其中磷脂双层的囊泡将膜递送至任意底物。然而，该方法对脂质体的大小和组成的变化以及表面的物理化学性质敏感。虽然尚未引入关于脂质体粘附，破裂，塌陷和随之而来的融合成双层膜的统一理论，但我们提出了一项实证研究，该研究遵循了从脂质体前体到沉积过程的膜形成过程，以及所得产物的结构表征。膜，用于代表性的哺乳动物（DMPC）和类细菌（DMPC：DMPG 4：1）膜，在羧酸封端的自组装单层膜上，可以很容易地在各种基底上形成膜，因此可用于制作膜沉积基底独立。

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