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Preparation of Zr4+ Affinity Column Based on Poly(styrene-co-glycidyl methacrylate) Microsphere for Phosphoprotein Isolation

机译:基于聚苯乙烯-甲基丙烯酸缩水甘油酯微球的Zr4 +亲和柱的制备

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1.Introduction The preparation methods for polymeric microspheres have been investigated for many years Dispersion and emulsion polymerizations are commonly used for the preparation of polymeric microspheres. Polymer microspheres are applied a wide field, for example, immunoassay, cell labeling, latex reagent, drug carrier, and column packing material for liquid chromatography [4-5]. The application field can be expanded by adding functional group on polymeric microspheres. Copolymerization is one of the methods for introduction of functional group on polymer microspheres. Glycidyl methacrylate (GMA) is one of the monomers which are easily modified into various functional groups. After GMA is polymerized, the epoxy groups of poly-GMA are changeable to alcohols [10] amines [6] phosohonic acid [7] sulfonic acid [8], etc.
机译:1引言对聚合物微球的制备方法进行了多年研究,分散和乳液聚合通常用于制备聚合物微球。聚合物微球的应用广泛,例如免疫分析,细胞标记,乳胶试剂,药物载体和用于液相色谱的柱填充材料[4-5]。通过在聚合物微球上添加官能团可以扩大应用领域。共聚是在聚合物微球上引入官能团的方法之一。甲基丙烯酸缩水甘油酯(GMA)是易于修饰成各种官能团的单体之一。 GMA聚合后,聚GMA的环氧基可转变为醇[10]胺[6]膦酸[7]磺酸[8]等。

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