• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文会议>2011 International Conference on New Technology of Agricultural Engineering >Identify of Rice Resistance to Blast (Magnaporthe grise) with SSR Markers
【24h】

Identify of Rice Resistance to Blast (Magnaporthe grise) with SSR Markers

机译：利用SSR标记鉴定水稻抗稻瘟病（Magnaporthe grise）

获取原文
获取原文并翻译 | 示例
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The broad-spectrum blast resistance gene Pi-1, from the donor line Lac23, was introduced into a line Nyou69-1, which possessed great resilience but high susceptibility to rice blast. The F8 individuals used in this study consisted 297 plants derived from a successively self-crossed with the material (a cross between Nyou69-1 and Lac23). SSR markers near Pi-1 gene at the end of the 11th chromosome of rice were used to detect the polymorphism in Nyou69-1 and Lac23. Five pairs of core primers showed polymorphism between Nyou69-1 and Lac23 were selected. Detect the F8 individuals with these primers, compare the lab result with the field trial, verify the accuracy of SSR marker to identify the rice blast resistance in field individuals. The result showed that °there was an apparent change in the phenomena of a gene recombination between markers and the Pi-1 caused by the great self-cross. In the lab trial, RM6293 got the highest accuracy rate among the 5 pairs of core primers, which was the most efficient single primers. Its result contained 22 individuals (53.66% in the positive bands) getting the property of . resistance to blast(the standard according to the result of field trial). Evaluating the different combinations of primers from the core primers indicated that RM6094/RM2136 was very efficient in identifying those F8 cultivars. 28 cultivars of F8 colony, representing 10 materials with the grade I- rice blast resistance, 12 materials with the grade III rice blast resistance, 6 materials with the grade V rice blast resistance, showed the positive strip in the testing. 14 materials with grade 1,30 materials with grade III and 19 materials with grade V have rice blast resistance in the field trial. Its accuracy rate reaches 73.68%. The result shows the elevation of the accuracy rate depend on the quantity of the primer combination. The more primers. form_ the combination, the higher accuracy rate it represent. Select three primers from the five core primers arbitrarily as a combination to detect the rice blast resistance, no fake positive materials have been detected, what is more, most of the materials selected by the three primers combination are grade I of the rice blast resistance, only 1 material is grade III of the rice blast resistance and no one is grade V of the rice blast resistance. The evidence proves that materials with high blast resistance were selected by the core primer combination efficiently. Some of the rice blast resistance materials be selected as the negative in the lab trial is insufficient through this research.
机译：来自供体系Lac23的广谱抗稻瘟病基因Pi-1被导入到Nyou69-1品系中，该品系具有很高的适应性，但对稻瘟病的敏感性很高。在这项研究中使用的F8个体由297种植物组成，这些植物源于与该材料的连续自交（Nyou69-1和Lac23之间的杂交）。用水稻第11号染色体末端的Pi-1基因附近的SSR标记检测Nyou69-1和Lac23的多态性。五对核心引物显示出Nyou69-1和Lac23之间的多态性。用这些引物检测F8个体，将实验室结果与田间试验进行比较，验证SSR标记的准确性，以鉴定田间个体的稻瘟病抗性。结果表明，由于巨大的自交，标记和Pi-1之间的基因重组现象有明显的变化。在实验室试验中，RM6293在5对核心引物中获得了最高的准确率，这是最有效的单引物。其结果包含22个个体（阳性带中53.66％），具有的性质。抗爆炸性（根据田间试验结果为标准）。从核心引物评估引物的不同组合表明，RM6094 / RM2136在鉴定那些F8品种方面非常有效。 F8菌落的28个品种，分别代表10种抗I级稻瘟病材料，12种抗III级稻瘟病材料，6种抗V级稻瘟病材料，在试验中显示出阳性条带。在田间试验中，14种1,3级材料和III级19种材料具有抗稻瘟病性。准确率达到73.68％。结果表明准确率的提高取决于引物组合的数量。底漆越多。 form_组合，表示的准确率更高。从五个核心引物中任意选择三个引物作为组合来检测稻瘟病抗性，未检测到假阳性材料，而且，通过这三个引物组合选择的大多数材料均为稻瘟病抗性I级，仅一种材料是抗稻瘟病的III级，而没有一种材料是抗稻瘟病的V级。证据证明，通过核心底漆组合可以有效地选择具有高抗爆炸性的材料。通过这项研究，一些耐稻瘟病材料被选为实验室试验的阴性材料是不够的。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号