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首页> 外文会议>2011 International Conference on New Technology of Agricultural Engineering >Comparative research on two kinds of amperometric immunosensor for detection of carbofuran residues
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Comparative research on two kinds of amperometric immunosensor for detection of carbofuran residues

机译：两种用于检测呋喃丹残留的安培免疫传感器的比较研究

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摘要

In this study, two kinds of novel label-free amperometric immnnosensor for the direct detection of carbofuran was developed using layer-by-layer assembly and sol-gel technology, respectively. In one hand" anti-carbofuran monoclonal antibodies were immobilized on the gold electrode (GE) surface which was modified with multilayers of L-cysteinewas, gold nanoparticles (nano-Au) and horseradish peroxidase (HRP) through layer-by-layer assembly technology, in the other hand, anti-carbofuran monoclonal antibodies were immobilized on the surface of the glassy carbon electrode (GCE) based -on sol-gel technology to prepare non-labeled electrochemical immunosensor for the detection of carbofuran. The conformational properties of the immunosensor were characterized using electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV) and a current response curve. Effects of experimental variables, such as concentration of antibody solution, pH of working buffer and incubation time, were studied in detail for optimization of analytical performance. Under optimal conditions, the current response was proportional to the concentration of carbofuran.
机译：在这项研究中，分别使用逐层组装和溶胶-凝胶技术，开发了两种直接检测卡百威的新型无标记安培免疫传感器。一方面，将“抗卡呋喃单克隆抗体”固定在金电极表面上，并通过逐层组装技术修饰了半胱氨酸，金纳米颗粒（纳米金）和辣根过氧化物酶（HRP）的多层膜。另一方面，基于溶胶-凝胶技术，将抗卡呋喃单克隆抗体固定在玻璃碳电极（GCE）的表面上，制备了用于检测呋喃丹的非标记电化学免疫传感器。使用电化学阻抗谱（EIS），循环伏安法（CV）和电流响应曲线对样品进行表征，详细研究了实验变量（如抗体溶液的浓度，工作缓冲液的pH值和孵育时间）的影响，以优化分析性能在最佳条件下，电流响应与呋喃丹的浓度成正比。

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