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Optimum Parameters of Preparation of Epidermal Cell Protoplasts within the Allium Cepa L

机译:洋葱头皮内表皮细胞原生质体制备的最佳参数

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摘要

Objective to optimize the- isolation condition of Epidermal Cell Protoplasts within the Allium cepa L. Methods According, to orthogonal experiment design L25 (56).of enzyme, osmotic pressure stabilizer(DMannitol), pH, enzymolysis time, we got the optimal isolation condition by the number of protoplasts. Results The optimal isolation condition is showed in results: enzymolysis time 2h, pH 5.5, D-Mannitol 0.6 mol/L, 1.5% cellulose and 0.1% pectolyase and 0.1% BSA, temperature 28 ℃.It also means that optimal isolation condition is D5B3QA2. Conclusion The optimal isolation condition is viable. .
机译:目的优化洋葱中表皮细胞原生质体的分离条件。方法根据正交试验设计L25(56)。对酶,渗透压稳定剂(DMannitol),pH,酶解时间进行优化,得到最佳分离条件。通过原生质体的数量。结果结果表明,最佳分离条件为:酶解时间2h,pH 5.5,D-甘露醇0.6 mol / L,1.5%纤维素和0.1%果胶酶和0.1%BSA,温度28℃,最佳分离条件为D5B3QA2。 。结论最佳隔离条件是可行的。 。

著录项

  • 来源
  • 会议地点 Zibo(CN)
  • 作者单位

    Key Laboratory of Modern Agricultural Equipment and Technology, Ministry of Education Jiangsu Province, Jiangsu University Zhenjiang, China;

    Key Laboratory of Modern Agricultural Equipment and Technology, Ministry of Education Jiangsu Province, Jiangsu University Zhenjiang, China;

    Key Laboratory of Modern Agricultural Equipment and Technology, Ministry of Education Jiangsu Province, Jiangsu University Zhenjiang, China;

    Key Laboratory of Modern Agricultural Equipment and Technology, Ministry of Education Jiangsu Province, Jiangsu University Zhenjiang, China;

  • 会议组织
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 农业工程;
  • 关键词

    Allium cepa L; Protoplast; Preparation; Orthogonal experiment;

    机译:葱原生质体制备;正交实验;

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