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首页> 外文会议>2012 12th IEEE International Conference on Nanotechnology. >Quantum dot electrophoretic mobility shift assay and its application to the measurement of exonuclease activity
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Quantum dot electrophoretic mobility shift assay and its application to the measurement of exonuclease activity

机译：量子点电泳迁移率变动分析及其在核酸外切酶活性测量中的应用

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摘要

We have discovered a phenomenon where the electrophoretic mobility of a QD-DNA nanoassembly can be precisely and predictably tuned by the level of surface DNA conjugation. By using streptavidin-coated quantum dots (QDs) as nanotethers to gather biotin-labeled DNA into electrophoretic nanoassemblies, the QD surface charge is modulated and transformed into electrophoretic mobility shifts using standard agarose gel electrophoresis. Based on this unique property, we have developed a quantification nanoassay termed as quantum dot electrophoretic mobility assay, or QEMSA. QEMSA demonstrates ultrahigh resolution in quantification, capable of differentiating ∼9% difference in DNA quantity. In this report, we further present a new analytical model that better describe the QEMSA process by taking into account the intrinsic charges carried by the quantum dot. We also extend the applications of QEMSA to measure the enzyme activity of exonuclease I.
机译：我们发现了一种现象，其中QD-DNA纳米组件的电泳迁移率可以通过表面DNA缀合的水平精确且可预测地调整。通过使用链霉亲和素包被的量子点（QD）作为纳米束将生物素标记的DNA收集到电泳纳米组件中，使用标准的琼脂糖凝胶电泳对QD表面电荷进行调制并转化为电泳迁移率移位。基于这种独特的性质，我们开发了一种定量纳米测定法，称为量子点电泳迁移率测定法或QEMSA。 QEMSA在定量方面显示出超高分辨率，能够区分约9％的DNA量差异。在此报告中，我们进一步提出了一种新的分析模型，该模型通过考虑量子点携带的固有电荷来更好地描述QEMSA过程。我们还扩展了QEMSA的应用，以测量核酸外切酶I的酶活性。

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