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首页> 外文会议>BMEI 2012;International Conference on Biomedical Engineering and Informatics >Experimental study of histotripsy in ex vivo bovine liver tissue exposed to pulsed high intensity focused ultrasound
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Experimental study of histotripsy in ex vivo bovine liver tissue exposed to pulsed high intensity focused ultrasound

机译：脉冲高强度聚焦超声暴露离体牛肝组织中组织变性的实验研究

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High intensity focused ultrasound (HIFU) has been used in clinical treatment for a variety of diseases, and achieved good results. Former related researches are mostly focused on either consecutive HIFU or HIFU with a low duty cycle, which is similar to the damage caused by consecutive HIFU mainly through thermal effect. However, there are few researches on tissue damage caused by non-thermal effect. Actually, the cavitation effect of HIFU can also cause serious tissue damage. So, to avoid the thermal effect caused by HIFU irradiation, this experiment will use high intensity HIFU of low duty cycle to irradiate bovine liver tissue in vitro, so as to make use of its non-thermal effect, especially the tissue damage directly caused by cavitation effect. A Commercial HIFU tumor therapeutic system, with 500W sound power, 4Hz pulse repetition rate and 1∼4% duty cycle parameter, is used to irradiate the degassed fresh bovine liver tissue, with a depth of 20 mm. During the experiment, the temperature of focal area is measured by thermocouple, and the cavitation activity is analyzed based on data gained by passive cavitation acquisition system. Then the irradiated bovine liver tissues in vitro were sliced into 1∼2 mm pieces to observe its damage form, and get the specimen for H&E staining to see the result of damaged tissue. After the experiment, it is discovered that the damage caused by irradiation of high intensive HIFU of low duty ratio is a cavernous injury without content (the content can be liquid), rather than containing necrotic tissue coagulation necrosis. What's more, during the experiment, the temperatures in focal region are 41.19±1.42°C, 45.73±1.92°C, 53.07±2.09°C respectively, no more than 56°C. If the duty ratio is higher (4%), the tissue damage caused by the irradiation of pHIFU will be coagulation necrosis, which is the same result caused by the irradiation of traditional cHIFU, a- d the temperature in the focal region is 64.13±2.56°C, surpassing 56°C. Cavitation can be clearly observed in all irradiation. After H&E staining, there is no intact cell at the edge of the visible hole-shape damage under microscope, cell nucleus vanishing and cytoplasmic homogeneously forming. We can conclude that: the irradiation of high intensive HIFU of low duty ratio can cause tissue damage, which is different from coagulation necrosis of the cavernous injury, and the damage formation involves cavitation. The result of this experiment implies that HIFU cavitation damage tissue can be a new method of treatment in the future.
机译：高强度聚焦超声（HIFU）已用于多种疾病的临床治疗，并取得了良好的效果。以前的相关研究主要集中在连续HIFU或低占空比的HIFU上，这与连续HIFU主要通过热效应造成的损害相似。然而，关于非热效应引起的组织损伤的研究很少。实际上，HIFU的空化作用也会引起严重的组织损伤。因此，为避免HIFU辐照引起的热效应，本实验将采用低占空比的高强度HIFU体外辐照牛肝组织，以利用其非热效应，特别是直接由HIFU辐照引起的组织损伤。空化作用。使用具有500W声功率，4Hz脉冲重复频率和1-4％占空比参数的商业HIFU肿瘤治疗系统照射深度为20 mm的脱气新鲜牛肝组织。在实验过程中，通过热电偶测量焦点区域的温度，并基于被动气蚀采集系统获得的数据分析气蚀活动。然后将体外辐照过的牛肝组织切成1-2mm的薄片，观察其损伤形式，并进行H＆E染色标本，观察损伤组织的结果。经过实验，发现由低占空比高强度HIFU辐照引起的损伤是无内容物的海绵状损伤（内容物可以是液体），而不是坏死组织凝结坏死。而且，在实验过程中，焦点区域的温度分别为41.19±1.42℃，45.73±1.92℃，53.07±2.09℃，不超过56℃。如果占空比较高（4％），则pHIFU辐照引起的组织损伤将是凝结坏死，这与传统cHIFU辐照引起的结果相同，并且焦点区域的温度为64.13± 2.56°C，超过56°C。在所有照射下都可以清楚地观察到气蚀现象。 H＆E染色后，在显微镜下可见孔形损伤的边缘没有完整的细胞，细胞核消失，细胞质均匀形成。我们可以得出以下结论：低强度高强度HIFU的照射会引起组织损伤，这与海绵体损伤的凝结坏死不同，损伤形成涉及空化。该实验的结果表明，HIFU空化损伤组织可能是未来的一种新治疗方法。

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