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Molecular analysis of the duck plague virus US10 gene and its encoding protein

机译:鸭瘟US10基因及其编码蛋白的分子分析

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In the past years, a number of conventional and advanced bioinformatics methods have been employed for predicting gene structure and function. Here, we used many bioinformatics softwares to analyse the duck plague virus US10 gene (GenBank accession number: EU195084). The results indicated that this gene encoded an estimated 322 putative protein, contained the conserved domain of the Gene66 (IR5) protein. The protein found many functional sites, but without any transmembrane region and signal peptide. Secondary structure prediction showed that random coil (52.48%) and alpha helix (44.10%) had a high proportion. Analysis of the antigenicity indicated that in the position of 28–44, 100–120 and 302–322 amino acids distributed the most likely epitopes. Phylogenetic tree of the amino acids sequences showed this gene had a closer evolutionary relationship with Mardivirus. These results provided rational data to elucidate biological function and physiological features of the gene and its encoding protein.
机译:在过去的几年中,许多常规和先进的生物信息学方法已被用于预测基因的结构和功能。在这里,我们使用了许多生物信息学软件来分析鸭瘟病毒US10基因(GenBank登录号:EU195084)。结果表明,该基因编码了估计的322个推定蛋白质,其中包含Gene66(IR5)蛋白质的保守结构域。该蛋白发现了许多功能位点,但没有任何跨膜区和信号肽。二级结构预测表明,无规卷曲(52.48%)和α螺旋(44.10%)具有很高的比例。抗原性分析表明,在28–44、100–120和302–322氨基酸位置分布了最可能的表位。氨基酸序列的系统进化树显示该基因与狂欢病毒具有更紧密的进化关系。这些结果提供了合理的数据,以阐明该基因及其编码蛋白的生物学功能和生理特征。

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