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首页> 外文会议>2013 ICME International Conference on Complex Medical Engineering >Differential proteome of the striatum from A30P #x03B1;-Synuclein transgenic mouse model of parkinson's disease
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Differential proteome of the striatum from A30P #x03B1;-Synuclein transgenic mouse model of parkinson's disease

机译：帕金森氏病A30Pα-突触核蛋白转基因小鼠模型纹状体差异蛋白质组

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摘要

Parkinson's disease (PD) is a multifactorial, neurodegenerative disease where etiopathogenesis are not fully understood. Mutations in α-Synuclein (α-Syn) were the first genetic defect linked to PD. They are deposited in Lewy bodies (LBs) characteristic for PD. Some experiments had showed that A30P mutant a-Syn have high toxicity than wide-type α-Syn. Here we used A30Pα-Syn transgenic mice model to analysed proteome changes of the striatum 11 months after the birth. Striata were removed and after digesting the proteins we used isotope labelling method to mark different group of peptides. Strong-cation exchange (SCX) liquid chromatography (LC) was integrated with peptide separation as the first dimension of the two-dimensional LC tandem mass spectrometry workflow. In this work, electrospray ionization (ESI) quadrupole time-of-flight (QTOF) mass spectrometer was explored as a means of detecting the Ms/Ms spectrogram. Agilent Spectrum Mill software was used to analysed the results. A total of 660 proteins were quantified. 280 proteins were down-regulated and 77 proteins were up-regulated.
机译：帕金森氏病（PD）是一种多因素的神经退行性疾病，其病因尚未完全明了。 α-突触核蛋白（α-Syn）的突变是与PD相关的第一个遗传缺陷。它们沉积在PD的路易体（LB）中。一些实验表明，A30P突变体a-Syn具有比宽型α-Syn高的毒性。在这里，我们使用A30Pα-Syn转基因小鼠模型分析了出生后11个月纹状体的蛋白质组变化。去除纹状体，消化蛋白质后，我们使用同位素标记法标记不同组的肽。强阳离子交换（SCX）液相色谱（LC）与肽分离集成在一起，是二维LC串联质谱工作流程的第一维。在这项工作中，探索了电喷雾电离（ESI）四极杆飞行时间（QTOF）质谱仪，作为检测Ms / Ms谱图的一种手段。使用Agilent Spectrum Mill软件分析结果。总共定量了660种蛋白质。 280种蛋白被下调，而77种蛋白被上调。

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