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首页> 外文会议>2013 ICME International Conference on Complex Medical Engineering >Protein profiling of striatum and substantia nigra from hemiparkinsonian rat model by offgel-HPLC-MS/MS
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Protein profiling of striatum and substantia nigra from hemiparkinsonian rat model by offgel-HPLC-MS/MS

机译：用offgel-HPLC-MS / MS对半帕金森病大鼠模型的纹状体和黑质进行蛋白质分析

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摘要

Parkinson's disease (PD) is a common neurodegenerative disease. The prevalence of PD among people over 65 years old is about 2 % and it is induced by multifactor including age, gene and environment. Recently, proteomics are increasingly being applied to identify biomarkers for disease. In this study, unilateral 6-hydroxydopamine (6-OHDA) in striatum of PD rat model was established first and demonstrated by motor behavior and immunocytochemistry of tyrosine hydroxylase staining. And then offgel as the first dimensional separation method combined with HPLC-MS/MS was used to analyze protein profiling of striatum and substantia nigra from PD rat. In striatum and substantia nigra, there were 858 and 751 proteins identified, respectively and 381 proteins were both in the two regions. GO (Gene Ontology) and KEGG (Kyoto Encyclopedia of Genes and Genomes) annotation revealed that these proteins were related to metabolism, apoptosis and signal transduction. Additionally, several PD related proteins were also involved in this study including ubiquitin carboxyl-terminal hydrolase isozyme L1 (UchI1), heat shock proteins and 14-3-3 proteins. These investigations would provide protein identification basis for PD pathogenesis and be helpful for further study for biomarker discovery.
机译：帕金森氏病（PD）是一种常见的神经退行性疾病。 65岁以上人群中PD的患病率约为2％，是由年龄，基因和环境等多种因素引起的。近来，蛋白质组学被越来越多地用于识别疾病的生物标记。本研究首先建立了PD大鼠模型纹状体中的单侧6-羟基多巴胺（6-OHDA），并通过酪氨酸羟化酶染色的运动行为和免疫细胞化学进行了证明。然后将Offgel作为一维分离方法，并与HPLC-MS / MS相结合，用于分析PD大鼠纹状体和黑质的蛋白质谱。在纹状体和黑质中，分别鉴定出858和751种蛋白质，并且在这两个区域中均鉴定出381种蛋白质。 GO（基因本体论）和KEGG（京都基因与基因组百科全书）注释表明，这些蛋白质与代谢，细胞凋亡和信号转导有关。此外，一些与PD相关的蛋白也参与了这项研究，包括泛素羧基末端水解酶同工酶L1（UchI1），热休克蛋白和14-3-3蛋白。这些研究将为PD发病机理提供蛋白质鉴定基础，并有助于进一步研究生物标志物的发现。

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