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首页> 外文会议>2014 International Symposium on Optomechatronic Technologies >Interferometric Focusing of Excitation Light onto a Guide-Star
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Interferometric Focusing of Excitation Light onto a Guide-Star

机译：激发光在引导星上的干涉聚焦

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Optical microscopy allows noninvasive imaging of biological tissues at a sub cellular level. However, the optimal performance of the microscope is hard to achieve because of aberrations induced from tissues. The shallow penetration depth and degraded resolution provide a limited degree of information for biologists. In order to compensate for aberrations, adaptive optics with direct wave front sensing, where guide-stars are used for wave front measurement, has been applied in microscopy. The scattering effect limits the intensity of a guide-star and hence reduces the signal to noise ratio of the wave front measurement. In this paper, we propose to use interferometric focusing of excitation light onto a guide-star embedded deeply in tissue to increase its fluorescence intensity, thus overcoming the signal loss caused by scattering. With interferometric focusing of light, we increase the signal to noise ratio of the laser guide-star through scattering tissue by more than two times as well as potentially extending the thickness of tissue that can be corrected using AO microscopy.
机译：光学显微镜允许在亚细胞水平上对生物组织进行无创成像。但是，由于组织引起的像差，难以实现显微镜的最佳性能。浅穿透深度和降低的分辨率为生物学家提供了有限程度的信息。为了补偿像差，在显微镜中采用了具有直接波前感测的自适应光学器件，其中使用了引导星进行波前测量。散射效应限制了导星的强度，因此降低了波阵面测量的信噪比。在本文中，我们建议使用干涉光将激发光聚焦到深埋在组织中的导星上，以增加其荧光强度，从而克服由散射引起的信号损失。通过光的干涉测量聚焦，我们可以使通过散射组织的激光导星的信噪比提高两倍以上，并潜在地扩展了可以使用AO显微镜校正的组织厚度。

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