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首页> 外文会议>2014 International Symposium on Optomechatronic Technologies >Optoelectrokinetically-Enabled Signal Enhancement for a Bead-Based FRET Fluorescence Immunoassay
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Optoelectrokinetically-Enabled Signal Enhancement for a Bead-Based FRET Fluorescence Immunoassay

机译：基于磁珠的FRET荧光免疫测定的光电动信号增强

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摘要

Bead-based immunoassay has been growing as a promising technology in the point-of-care diagnostics due to great flexibility. For samples with dilute analytes, the colloidal particles can concentrate dispersed analytes and act as carriers for advanced particle manipulation. To realize a rapid in-situ immunoassay, Firster resonance energy transfer (FRET) was used herein to ensure only the target antigen lipocalin 1 (LCN1) to be detected. The measurement was made in an aqueous droplet sandwiched between two parallel electrodes. In addition, an optoelectrokinetic technique was used to further concentrate the functionalized particles to enhance the FRET fluorescent signal. The detection was usually completed within several tens of seconds. A comparison between the based-based diagnostics coupled with the optoelectrokinetic technique and a conventional ELISA reader showed nearly 100-fold improvement of the bio-signal was made. The technique is expected to bring hopes for diagnosing diseases with dilute biomarkers in the early stage.
机译：由于具有很大的灵活性，基于珠子的免疫测定法已经成为即时诊断中一种有前途的技术。对于分析物稀的样品，胶体颗粒可以浓缩分散的分析物，并充当高级颗粒处理的载体。为了实现快速的原位免疫测定，本文使用了第一共振能量转移（FRET）以确保仅检测到目标抗原脂蛋白1（LCN1）。在夹在两个平行电极之间的水滴中进行测量。另外，使用光电动力学技术进一步浓缩功能化颗粒以增强FRET荧光信号。检测通常在几十秒内完成。将基于基础的诊断与光电动技术相结合，并与传统的ELISA读数器进行比较，结果表明生物信号得到了近100倍的改善。该技术有望为早期用稀薄生物标志物诊断疾病带来希望。

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