Extraction by Reversed Micelles of the Intracellular Enzyme Xylose Reductase

机译：反向胶束提取胞内酶木糖还原酶

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Xylose reductase enzyme (EC 1.1.1.21) produced by Candida guilliermondii in sugarcane bagasse was extracted by reversed micelles of N-benzyl-N-dodecyl-N-frz's (2-hydroxyethyl) ammonium chloride cationic surfactant. An experimental design was employed to evaluate the influences of the following factors on the enzyme extraction: temperature, cosolvent, and surfactant concentration. A model was used to represent the enzyme recovery and fit of the experimental data. The extraction yielded a total recovery of 130%, and the purity increased 4.8-fold. This study demonstrates that liquid-liquid extraction by reversed micelles is a process able to recover and increase the enzymatic activity and purity of XR produced by C. guilliermondii.

机译：用N-苄基-N-十二烷基-N-frz的（2-羟乙基）氯化铵阳离子表面活性剂的反胶束提取古迪假丝酵母在甘蔗渣中产生的木糖还原酶（EC 1.1.1.21）。实验设计用于评估以下因素对酶提取的影响：温度，助溶剂和表面活性剂浓度。使用模型表示酶回收率和实验数据的拟合度。提取后的总回收率为130％，纯度提高了4.8倍。这项研究表明，通过反胶束进行液-液萃取是一个能够回收并提高古氏梭状芽胞杆菌产生的XR的酶促活性和纯度的过程。

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来源
《22th Symposium on Biotechnology for Fuels and Chemical, 22th, May 7-11, 2000, Gatlinburg, Tennessee》|2000年|p.753-759|共7页
会议地点 Gatlinburg TN(US)
作者
ELY V. CoRTEZ; MARIA DAS GRAcAS DE ALMEIDA FELIPE; INES C. ROBERTO; ADALBERTO PESSOA; MlCHELE VlTOLO;
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作者单位

Faculdade de Ciencias Farmaceuticas/USP, PO Box 66083, Sao Paulo, SP, Brazil;

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会议组织
原文格式 PDF
正文语种 eng
中图分类生物化学;
关键词
reversed micelles; xylose reductase; liquid-liquid extraction;

机译：反胶束木糖还原酶液液萃取;

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专利

1. Extraction by Reversed Micelles of the Intracellular Enzyme Xylose Reductase [J] . ElyV.Cortez, Maria das Gracas de Almeida Felipe, Ines C.Roberto, Applied biochemistry and biotechnology, Part A. enzyme engineering and biotechnology . 2001,第0期

机译：反向胶束提取胞内酶木糖还原酶
2. Optimized extraction by cetyl trimethyl ammonium bromide reversed micelles of xylose reductase and xylitol dehydrogenase from Candida guilliermondii homogenate [J] . Ely Vieira Cortez, Adalberto Pessoa Jr., Maria das Gracas de Almeida Felipe, Journal of chromatography, B. Analytical technologies in the biomedical and life sciences . 2004,第1期

机译：十六烷基三甲基溴化铵反胶束从古德氏假丝酵母匀浆中木糖还原酶和木糖醇脱氢酶的优化提取
3. Preliminary Characterization of Xylose Reductase Partially Purified by Reversed Micelles from Candida tropicalis IEC5-ITV, an Indigenous Xylitol-Producing Strain [J] . Yolanda Cocotle-Ronzon, Marisol Zendejas-Zaldo, Micloth López del Castillo-Lozano, Advances in Chemical Engineering and Science . 2012,第1期

机译：木糖还原酶的初步表征，部分是由热带假丝酵母IEC5-ITV（一种产木糖醇的菌株）的反向胶束纯化的
4. Extraction by Reversed Micelles of the Intracellular Enzyme Xylose Reductase [C] . MlCHELE VlTOLO, ADALBERTO PESSOA, INES C. ROBERTO, Symposium on biotechnology for fuels and chemicals . 2001

机译：细胞内酶木糖还原酶的反向胶束提取
5. Structural studies of three NAD(P)H-dependent enzymes involved in sugar metabolism: Mannitol dehydrogenase, xylose reductase, and lactate dehydrogenase. [D] . Kavanagh, Kathryn Louise. 2003

机译：糖代谢中涉及的三种NAD（P）H依赖酶的结构研究：甘露醇脱氢酶，木糖还原酶和乳酸脱氢酶。
6. NAD(P)H-dependent aldose reductase from the xylose-assimilating yeast Candida tenuis. Isolation characterization and biochemical properties of the enzyme. [O] . W Neuhauser, D Haltrich, K D Kulbe, 1997

机译：NAD（P）H依赖的木糖同化酵母假丝酵母的醛糖还原酶。酶的分离表征和生化特性。
7. Preliminary Characterization of Xylose Reductase Partially Purified by Reversed Micelles from <i>Candida tropicalis</i> IEC5-ITV, an Indigenous Xylitol-Producing Strain [O] . Micloth López del Castillo-Lozano, Marisol Zendejas-Zaldo, Yolanda Cocotle-Ronzon, 2011

机译：木糖醇还原菌株 Candida Tropicalis IEC5-ITV反向胶束部分纯化的木糖还原酶的初步表征

1. 黑曲霉中提取葡萄糖氧化酶的探索：Ⅱ.用反向胶束法提取？… [J] . 杨惠英 ,李弘毅 . 中山大学学报：自然科学版 . 1998,第006期

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3. 转木糖还原酶基因XYL1酿酒酵母的构建及产木糖醇能力研究 [J] . 王凤梅 ,张邦建 ,岳泰新 . 中国酿造 . 2018,第012期

4. 共表达木聚糖酶和木糖还原酶生产木糖醇的研究 [J] . 王晓霞 ,郑晨娜 ,王飞飞 . 食品与发酵工业 . 2007,第004期

5. 稳定高效表达木糖还原酶基因工业酿酒酵母的构建及木糖醇发酵的初步研究 [J] . 李敏 ,马涛 ,生举正 . 食品与发酵工业 . 2006,第001期

6. 运动发酵单胞菌木糖醇形成机制与葡萄糖—果糖氧化还原酶的双功能作用 [C] . 张晓梅 ,刘巍峰 ,陈冠军 . 第七届中国酶工程学术研讨会 . 2009

7. 木糖代谢关键酶的基因克隆及木糖还原酶的分子改造 [A] . 曾琦锴 . 2009

1. 过表达木糖还原酶、木糖醇脱氢酶和木酮糖激酶以促进木糖乙醇发酵的耐热酵母菌多形汉逊酵母的重组菌株 [P] . 中国专利： CN102046776B . 2013.07.31

2. 木糖还原酶的改造和木糖醇脱氢酶与木酮糖激酶的过表达促进耐热酵母菌多形汉逊酵母中的木糖乙醇发酵 [P] . 中国专利： CN102046776A . 2011-05-04

3. Method for controlling reverse micelle size in reverse micelle extraction system [P] . 外国专利： JP5382563B2 . 2014-01-08

机译：反胶束提取系统中反胶束大小的控制方法

4. METHOD FOR CONTROLLING REVERSED MICELLE SIZE IN REVERSED MICELLE EXTRACTION SYSTEM [P] . 外国专利： JP2007330874A . 2007-12-27

机译：反向胶束提取系统中反向胶束大小的控制方法

5. RECOMBINANT YEASTS CONTAINING THE DNA SEQUENCES CODING FOR XYLOSE REDUCTASE-AND XYLITOL DEHYDROGENASE ENZYMES [P] . 外国专利： CA2090122C . 2002-06-18

机译：含有木糖还原酶和木糖醇脱氢酶酶的DNA序列的重组酵母

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Extraction by Reversed Micelles of the Intracellular Enzyme Xylose Reductase

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