Fingerprinting Trichoderma reesei Hydrolases in a Commercial Cellulase Preparation

机译：商业纤维素酶制剂中的里氏木霉水解酶指纹图谱

获取原文

获取原文并翻译 | 示例

页面导航

摘要
著录项
相似文献
相关主题

摘要

Polysaccharide degrading enzymes from commercial T. reesei broth have been subjected to "fingerprint" analysis by high-resolution 2-D gel electro-phoresis. Forty-five spots from 11 X 25 cm Pharmacia gels have been analyzed by LC-MS/MS and the resulting peptide sequences were compared to existing databases. Understanding the roles and relationships of component enzymes from the T. reesei cellulase system acting on complex substrates is key to the development of efficient artificial cellulase systems for the conversion of lignocellulosic biomass to sugars. These studies suggest follow-on work comparing induced and noninduced T. reesei cells at the proteome level, which may elucidate substrate-specific gene regulation and response.

机译：来自商业里氏木霉肉汤的多糖降解酶已经通过高分辨率2-D凝胶电泳进行了“指纹”分析。通过LC-MS / MS分析了11 X 25 cm Pharmacia凝胶中的45个斑点，并将所得的肽序列与现有数据库进行了比较。理解里氏木霉纤维素酶系统中作用于复杂底物的组成酶的作用和关系是开发有效的人造纤维素酶系统以将木质纤维素生物质转化为糖的关键。这些研究表明，在蛋白质组水平上比较诱导的和未诱导的里氏木霉细胞的后续工作可阐明底物特异性基因的调控和应答。

著录项

来源
《22th Symposium on Biotechnology for Fuels and Chemical, 22th, May 7-11, 2000, Gatlinburg, Tennessee》|2000年|p.99-107|共9页
会议地点 Gatlinburg TN(US)
作者
T. B. VlNZANT; W. S. ADNEY; S. R. DECKER; J. O. BAKER; M. T. KlNTER; N. E. SHERMAN; J. W. FOX; M. E. HlMMEL;
展开▼
作者单位

Biotechnology Center for Fuels and Chemicals, National Renewable Energy Laboratory, Golden, CO 80401;

展开▼
会议组织
原文格式 PDF
正文语种 eng
中图分类生物化学;
关键词
cellulase; trichoderma reesei; two-dimensional gel electro-phoresis; liquid chromatography-mass spectrometry/mass spectrometry;

机译：纤维素酶里氏木霉二维凝胶电泳；液相色谱-质谱/质谱;

相似文献

外文文献
中文文献
专利

1. Fingerprinting Trichoderma reesei Hydrolases in a Commercial Cellulase Preparation [J] . T.B.Vinzant, W.S.Adney, S.R.Decker, Applied biochemistry and biotechnology, Part A. enzyme engineering and biotechnology . 2001,第0期

机译：商业纤维素酶制备中的里氏木霉水解酶指纹图谱
2. Precipitation of Trichoderma reesei commercial cellulase preparations under standard enzymatic hydrolysis conditions for lignocelluloses. [J] . Chylenski P, Felby C, Ostergaard Haven M, Biotechnology Letters . 2012,第8期

机译：在木质纤维素的标准酶水解条件下沉淀里氏木霉商业纤维素酶制剂。
3. Precipitation of Trichoderma reesei commercial cellulase preparations under standard enzymatic hydrolysis conditions for lignocelluloses [J] . Piotr Chylenski, Claus Felby, Mai Østergaard Haven, Biotechnology Letters . 2012,第8期

机译：在标准酶水解条件下木质纤维素的里氏木霉商业纤维素酶制剂的沉淀
4. Fingerprinting Trichoderma reesei Hydrolases in a Commercial Cellulase Preparation [C] . M. E. HlMMEL, J. W. FOX, N. E. SHERMAN, Symposium on biotechnology for fuels and chemicals . 2001

机译：在商业纤维素酶制剂中指纹Trichoderma Reesei水解酶
5. Cellulase production by Trichoderma reesei Rut C30. [D] . Lo, Chi-Ming. 2008

机译：里氏木霉Rut C30生产纤维素酶。
6. Enhanced Production of Trichoderma reesei Endoglucanases and Use of the New Cellulase Preparations in Producing the Stonewashed Effect on Denim Fabric [O] . Arja Miettinen-Oinonen, Pirkko Suominen 2002

机译：里氏木霉内切葡聚糖酶的产量提高以及新纤维素酶制剂在牛仔布上的水洗效果
7. Precipitation of Trichoderma reesei commercial cellulase preparations under standard enzymatic hydrolysis conditions for lignocelluloses [O] . Chylenski Piotr, Felby Claus, Haven Mai Østergaard, 2012

机译：在标准酶水解条件下木质纤维素的里氏木霉商业纤维素酶制剂的沉淀

Fingerprinting Trichoderma reesei Hydrolases in a Commercial Cellulase Preparation

摘要

著录项

相似文献

相关主题

期刊订阅