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首页> 外文会议>2nd International EUSPEN Conference on Precision Engineering Nanotechnology Vol.1, May 27th-31st, 2001, Turin, Italy >The Effect of the Stereo Focus Technique on the Three-Dimensional Imaging in the Confocal Scanning Microscopy
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The Effect of the Stereo Focus Technique on the Three-Dimensional Imaging in the Confocal Scanning Microscopy

机译：共聚焦扫描显微镜中立体聚焦技术对三维成像的影响

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This paper discusses one of the major error sources in the confocal scanning microscopy. In this paper, the laser drift effect on the three-dimensional surface imaging is demonstrated using the simulation. It is shown that the brightness drift of the laser can cause inaccurate surface geometry information. In addition, this paper discusses a two longitudinal focal points technique (the stereo focus technique). This study demonstrates that the stereo focus technique is more laser drift insensitive than conventional single detector techniques. The paper also shows that the stereo focus technique can improve the optical sectioning strength in the confocal microscope.
机译：本文讨论了共聚焦扫描显微镜的主要误差来源之一。本文通过仿真演示了激光漂移对三维表面成像的影响。结果表明，激光器的亮度漂​​移会导致不正确的表面几何信息。另外，本文讨论了两个纵向焦点技术（立体声焦点技术）。这项研究表明，与传统的单探测器技术相比，立体聚焦技术对激光漂移的敏感度更高。本文还表明，立体聚焦技术可以提高共聚焦显微镜的光学切片强度。

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