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首页> 外文会议>2nd Italian Conference on Sensors and Microsystems Artificial and Natural Perception Rome, Italy 3-5 February 1997 >Determination of Biogenic Amines with an Electrochemical Biosensor and Its Application in Fish Samples
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Determination of Biogenic Amines with an Electrochemical Biosensor and Its Application in Fish Samples

机译：电化学生物传感器测定生物胺及其在鱼类样品中的应用

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摘要

This paper describes the assembling and optimization of an electrochemical biosensor for the determination of biogenic amines commonly present in food products (putrescine, cadaverine, histamine, tyramine, spermidine, spermine, tryptamine) and its application to salted anchovy samples. Variations of the amine content in anchovies during ripening time were measured both with the biosensor and ion chromatography with integrated pulsed amperometric detection (IC-IPAD). The probe is based on a platinum electrode which senses the hydrogen peroxide produced by the reaction catalysed by the enzyme diamine oxidase (DAO) immobilized on the electrode surface. Parameters as enzyme immobilization and pH have been studied and optimized in order to obtain similar sensitivity for all the amines tested. The immobilization of the enzyme on a nylon-net membrane, using glutaraldehyde as cross-linking agent, and phosphate buffer at pH 8.0 were selected. The linear range common to the amines tested was observed from 1x10~(-6) mol/L to 5x10~(-5) mol/L. Underivatised biogenic amines such as putrescine, cadaverine, histamine, tyramine and spermidine were also detected with the IC-IPAD method. Changes in the concentration of biogenic amine content in salted anchovy samples measured with the biosensor and IC-IPAD methods, exhibited the same trend and demonstrated that the biosensor is an useful tool to monitor the variation of the total amine content in fish during storage.
机译：本文介绍了电化学生物传感器的组装和优化，用于测定食品中常见的生物胺（腐胺，尸胺，组胺，酪胺，亚精胺，亚精胺，色胺），并将其应用于盐腌的cho鱼样品中。使用生物传感器和带集成脉冲安培检测器的离子色谱法（IC-IPAD）测量了成熟期间凤尾鱼中胺含量的变化。该探针基于铂电极，该铂电极可感应由固定在电极表面的二胺氧化酶（DAO）催化的反应产生的过氧化氢。已对酶固定化和pH值等参数进行了研究和优化，以使所有被测胺获得相似的灵敏度。使用戊二醛作为交联剂，将酶固定在尼龙网膜上，并选择pH 8.0的磷酸盐缓冲液。从1x10〜（-6）mol / L到5x10〜（-5）mol / L可以观察到被测胺共有的线性范围。还使用IC-IPAD方法检测了未衍生的生物胺，例如腐胺，尸胺，组胺，酪胺和亚精胺。用生物传感器和IC-IPAD方法测量的盐腌cho鱼样品中生物胺含量的浓度变化，显示出相同的趋势，并表明该生物传感器是监测鱼类在储存过程中总胺含量变化的有用工具。

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