Study of Lactobacillus Acidophilus by Restriction Fragment Length Polymorphism (RFLP) Analysis

机译：通过限制性片段长度多态性（RFLP）分析研究嗜酸乳杆菌

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Lactobacillus acidophilus strain is one of the most commonly used probiotics. L. acidophilus is linked to decreased instances of vaginal yeast infection, gastrointestinal dysfunction and even boosting immune function. Common food sources of L. acidophilus include yogurt and fermented soy products, such as miso and tempeh. There are many species of Lactobacillus acidophilus bacteria that are found in a variety of environments, from dairy products to the human gastrointestinal tract (GI). Identification of L. acidophilus species from different sources is important that will supply their different products to contibute in medicinal and life sciences. Therefore, this study was to find the DNA profiles in Lactobacillus acidophilus ATCC 4356 and Lactobacillus acidophilus VTCC-B-871 by restriction fragment length polymorphism (RFLP) analysis. Besides, using a probe having 400 base pairs (bp) of minD gene from Lactobacillus rhamnosus JCM 1553, Southern blotting and hybridization were performed. There were a difference between two tested Lactobacillus acidophilus.

机译：嗜酸乳杆菌菌株是最常用的益生菌之一。嗜酸乳杆菌与减少阴道酵母菌感染，胃肠道功能障碍甚至增强免疫功能有关。嗜酸乳杆菌的常见食物来源包括酸奶和发酵的大豆产品，例如味o和豆eh。从乳制品到人类胃肠道（GI），在各种各样的环境中发现了许多嗜酸乳杆菌细菌。鉴定来自不同来源的嗜酸乳杆菌种类很重要，这将提供其不同的产品以在医学和生命科学中发挥作用。因此，本研究旨在通过限制性片段长度多态性（RFLP）分析找到嗜酸乳杆菌ATCC 4356和嗜酸乳杆菌VTCC-B-871中的DNA谱。此外，使用具有400个碱基对（bp）的鼠李糖乳杆菌JCM 1553的minD基因的探针，进行了Southern印迹和杂交。两种测试的嗜酸乳杆菌之间存在差异。

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来源
《4th International Conference on Biomedical Engineering in Vietnam》|2012年|195-197|共3页
会议地点 Chi Minh City(VN)
作者
H.K. Tu Nguyen; D. Ly Ha; T.T. Vinh Doan; T. Tu Quach; H. Kim Nguyen;
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作者单位

School of Biotechnology, International University, Hochiminh City National University;

Department of Reference Substances, Institute for Drug Quality Control, Hochiminh City;

School of Biotechnology, International University, Hochiminh City National University;

School of Biotechnology, International University, Hochiminh City National University;

The Retina Centers of Washington, VA;

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Lactobacillus acidophilus ATCC 4356; Lactobacillus acidophilus VTCC-B-871; restriction fragment length polymorphism (RFLP);

机译：嗜酸乳杆菌ATCC 4356;嗜酸乳杆菌VTCC-B-871;限制性片段长度多态性（RFLP）;

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Study of Lactobacillus Acidophilus by Restriction Fragment Length Polymorphism (RFLP) Analysis

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