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首页> 外文会议>55th International Astronautical Congress 2004 vol.3 >EVALUATION OF THE EFFECT OF CLINOSTAT ROTATION ON POLLEN GERMINATION AND TUBE DEVELOPMENT AS A TOOL FOR SELECTION OF PLANTS IN SPACE
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EVALUATION OF THE EFFECT OF CLINOSTAT ROTATION ON POLLEN GERMINATION AND TUBE DEVELOPMENT AS A TOOL FOR SELECTION OF PLANTS IN SPACE

机译：作为植物选择工具的恒温器旋转对花粉萌发和管子发育的影响的评价

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The choice of species and cultivar on which rely to sustain Close Loop Environmental Systems is generally approached by analysing the behaviour of plants in presence of stress (sporophytic selection). In this paper, we investigated the possibility to conduct the selection among genotypes in Space through the male gametophytic selection. Thus, we studied the effect of simulated microgravity on pollen germination and tube development of both woody and herbaceous crop species: Prunus armeniaca (apricot), Prunus dulcis (almond), Malus domestica (apple) and Vicia faba (broad bean). Pollen collected from just bloomed flowers was assessed for viability and incubated on the optimal growing medium in petri dishes both on a uni-axial clinostat and stationary in 1-g. Then, pollen was observed under a light microscope to detect percent germination and growth direction. Histochemical analyses were performed to verify the presence and distribution of storage substances. Moreover, specific staining and epifluorescent microscopy were applied to count nuclei, follow the migration of sperm cells and investigate the presence, size and morphology of callose plugs. Results showed that simulated microgravity affected pollen tube development. The different response showed by the various species indicate that male-gametophytic selection could be useful for the selection of plants in microgravity.
机译：通常通过分析在有胁迫的情况下植物的行为（孢子体选择）来选择维持闭环环境系统所依赖的物种和品种。在本文中，我们研究了通过雄配子体选择在空间中进行基因型选择的可能性。因此，我们研究了模拟微重力对木质和草本作物品种的花粉萌发和花粉管发育的影响：李子杏（杏），李子（杏仁），海棠（苹果）和蚕豆（蚕豆）。评估从刚盛开的花朵中收集的花粉的生存力，并在培养皿中的最佳生长培养基上以单轴斜率仪和固定在1-g中的温育。然后，在光学显微镜下观察花粉以检测发芽百分比和生长方向。进行了组织化学分析以验证存储物质的存在和分布。此外，应用特异性染色和落射荧光显微镜对细胞核进行计数，追踪精子细胞的迁移，并研究ose质栓的存在，大小和形态。结果表明，模拟微重力影响花粉管的发育。各种物种表现出的不同反应表明雄配子体的选择对于微重力条件下的植物选择可能是有用的。

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