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A host shift of diamondback moth from crucifers to peas:Life history traits and genetic mechanisms

机译:小菜蛾从十字花科到豌豆的宿主转移:生活史特征和遗传机制

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The diamondback moth(DBM)iS a well-known specialist on cruciferous plants.However,in 2001,a host shift to commercially-grown sugar pea was reported from Kenya. To study this phenomenon further,we compared five laboratory strains,including the pea-adapted strain from Kenya(DBM-P). In feeding trials on cabbage and pea,DBM-P larvae performed comparably well on the two hosts,whereas larvae of the other strains could not survive on pea. The genetic diversity within and between 10-12 individuals of the five strains was investigated using microsatellites and AFLPs.Statistical analyses of the AFLP data revealed hish genetic diversity between(FST=0.297)and within the strains. To study the inheritance of“pea-feeding”,we conducted single pair matings between DBM-P strain and a cabbage-adapted strain followed by a backcross to either the cabbage(BC Ⅰ)or the pea-adapted(BCⅡ)strain or by intercrossing the F1 to produce an F2. The results suggest recessive genes to be responsible for the trait“feeding ability on pea”.
机译:小菜蛾是著名的十字花科植物专家。但是,2001年,肯尼亚报道了一种向商业种植豌豆转变的宿主。为了进一步研究这种现象,我们比较了五个实验室菌株,包括肯尼亚的豌豆适应菌株(DBM-P)。在白菜和豌豆的饲喂试验中,DBM-P幼虫在两个寄主上的表现相当好,而其他菌株的幼虫无法在豌豆上存活。利用微卫星和AFLPs对5个菌株的10-12个个体之间及其之间的遗传多样性进行了研究。对AFLP数据的统计分析显示了(FST = 0.297)和菌株内部的his遗传多样性。为了研究“饲喂豌豆”的遗传,我们在DBM-P菌株和适应白菜的菌株之间进行单对交配,然后回交至甘蓝(BCⅠ)或适应豌豆(BCⅡ)的菌株或通过与F1交叉产生F2。结果表明隐性基因与“豌豆的摄食能力”有关。

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