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首页> 外文会议>64th Annual Convention of the Sugar Technologists' Association of India Aug 17-19, 2002 Cochin (Kerala) >MASS-CULTURE AND FORMULATION OF THREE ENTOMOGENOUS FUNGI, WITH SPECIAL REFERENCE TO BEAUVERIA BRONGNIARTII (SACC.) PETCH, AGAINST HOLOTRICHIA SERRATA F. (COLEOPTERA: SCARABAEIDAE)
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MASS-CULTURE AND FORMULATION OF THREE ENTOMOGENOUS FUNGI, WITH SPECIAL REFERENCE TO BEAUVERIA BRONGNIARTII (SACC.) PETCH, AGAINST HOLOTRICHIA SERRATA F. (COLEOPTERA: SCARABAEIDAE)

机译：三种昆虫真菌的质量培养和配方，特别是对白僵菌（Beauveria bronniartii）（SACC。）的宠物，对抗霍洛氏菌SERRATA F.（COLEOPTERA：SCARABAEIDAE）

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摘要

A technique for mass culturing three entomogenous fungi, namely a white grub isolate of Beauueria brongniartii (Sacc.) Petch, root borer isolate of Beauveria bassiana (Balsamo-Criv.) Vuill. and white grub isolate of Metarhizium anisopliae Metch on molasses yeast media was developed. Based on growth parameters, particularly spore production, molasses at 3% concentration was found to be suitable for mass culturing the three fungi. The optimum quantity of molasses broth in 1 liter polyvinyl containers was found to be 300 ml. In different multiple harvest regimes without replacement of media and re-inoculation of the fungus, highest spore production was obtained when two harvests, each with a 20 day incubation period, were made. Sulphur added to molasses media as a wettable form and CaSO_4, at the concentration contained in press-mud used as carrier material, did not affect growth parameters of the three fungi. Molasses broth media was less expensive than the standard media for culturing B. brongniartii. A formulation of the fungus cultured on molasses yeast broth was prepared using press-mud and lignite as carrier material. In a laboratory bioassay, press-mud formulation caused higher mortality of third instar grub than lignite formulation.
机译：大量培养三种昆虫致病真菌的技术，即白僵菌博古虫（Bauueria brongniartii）（Sacc。）Petch，球孢白僵菌（Beauveria bassiana）（Balsamo-Criv。）Vuill的根虫分离株。开发了在糖蜜酵母培养基上的Metarhizium anisopliae Metch的白色g分离株。基于生长参数，特别是孢子产生，发现3％浓度的糖蜜适合于大量培养这三种真菌。发现在1升聚乙烯容器中的糖蜜肉汤的最佳量为300毫升。在不同的多次收获方式中，无需更换培养基和重新接种真菌，当两次收获（每次孵化期为20天）时获得最高的孢子产量。以可湿性形式添加到糖蜜培养基中的硫和压榨泥中用作载体材料的浓度的CaSO_4不会影响这三种真菌的生长参数。糖蜜肉汤培养基比培养B. brongniartii的标准培养基便宜。使用压泥和褐煤作为载体材料，制备了在糖蜜酵母肉汤中培养的真菌制剂。在实验室生物测定中，压泥配方引起的第三龄g的死亡率高于褐煤配方。

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