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首页> 外文会议>The 6th China international conference on nanoscience and technology >A DNA Electrochemical Sensor with Poly-L-Lysine/Polyaniline Nanotubes Composite Film and Its Application for the Highly Sensitive EIS Detection of PAT Gene Fragment
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A DNA Electrochemical Sensor with Poly-L-Lysine/Polyaniline Nanotubes Composite Film and Its Application for the Highly Sensitive EIS Detection of PAT Gene Fragment

机译：聚-L-赖氨酸/聚苯胺纳米管复合膜的DNA电化学传感器及其在PAS基因片段高灵敏EIS检测中的应用

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摘要

A new DNA electrochemical biosensor was described for detection of the PAT gene fragment. Poly-L-lysine and polyaniline nanotube was mixed in terms of proper ratio. Then, the mixture was modified onto the CPE to form composite film. DNA probes with phosphate groups were easily immobilized on the poly-L-lysine film with positive charged interface due to the strong static affinity. Cyclic voltammetry and electrochemical impedance spectroscopy (EIS) were used to characterize the ssDNA immobilization and hybridization with its complementary DNA sequence by [Fe(CN)6]3-/4- as indicator. The conditions for preparing composite film, DNA immobilization and hybridization were optimized. The PAT gene fragment from phosphinothricin acetyltransferase gene was detected by this DNA electrochemical sensor. The dynamic detection range of the sensor to the PAT gene fragment was from 1.0×10-11 to 1.0×10-6 mol/L. A detection limit of 2.9×10-12 mol/L could be estimated.
机译：描述了一种新的DNA电化学生物传感器，用于检测PAT基因片段。按照适当的比例混合聚-L-赖氨酸和聚苯胺纳米管。然后，将混合物改性到CPE上以形成复合膜。具有很强的静态亲和力，带有磷酸基团的DNA探针很容易固定在带正电荷界面的聚L-赖氨酸膜上。以[Fe（CN）6] 3- / 4-为指示剂，利用循环伏安法和电化学阻抗谱（EIS）表征ssDNA的固定化及其与其互补DNA序列的杂交。优化了制备复合膜的条件，DNA固定化和杂交的条件。通过该DNA电化学传感器检测到了膦丝菌素乙酰转移酶基因的PAT基因片段。传感器对PAT基因片段的动态检测范围为1.0×10-11至1.0×10-6 mol / L。估计的检出限为2.9×10-12 mol / L。

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