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首页> 外文会议>The 6'th International Forum on Post-genome Technologies(6'IFPT)（第六届国际后基因组生命科学技术学术论坛） >COMPARISON OF THREE WHOLE GENOME AMPLIFICATION (WGA) METHODS OF GENOMIC DNA FROM ESCHERICHIA COLI
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COMPARISON OF THREE WHOLE GENOME AMPLIFICATION (WGA) METHODS OF GENOMIC DNA FROM ESCHERICHIA COLI

机译：大肠杆菌基因组DNA的三种全基因扩增（WGA）方法的比较

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摘要

In many large genetic studies, the amount of available DNA can be one of the criteria for selecting samples for study. Being well-known, DNA sample preparation is time-consuming and laborious, even it seems unanswered when the type and amount of sample available is a limiting factor, either due to degradation or low copy number. To solve these problems, Whole genome amplification (WGA) , based on DNA-strand displacement, is an option to amplify DNA in not-yet longer time reaction. The DNA amplification is non-PCR based and uses Φ29 DNA polymerase and modified random hexamer primers for unbiased whole genome amplificatioa There are various WGA kits which have been developed, for example the Repli-g Mini kit (Qiagen) and the GenomiPhi kit (GE Healthcare). So, it is of great important to select a perfect Kit suited for our research. In this study, after comparison of three WGA kits, there are the differences illustrated that the WGA product amplified from Escherichia coli genomic DNA is representative of the different amplification efficiency.
机译：在许多大型遗传研究中，可用DNA的量可能是选择要研究的样品的标准之一。众所周知，DNA样品的制备既费时又费力，即使由于降解或拷贝数低而使可用样品的类型和数量成为限制因素时，DNA样品的制备似乎也无法解决。为了解决这些问题，基于DNA链置换的全基因组扩增（WGA）是在尚未经过更长时间的反应中扩增DNA的一种选择。 DNA扩增是基于非PCR的，使用Φ29DNA聚合酶和修饰的随机六聚体引物进行无偏倚的全基因组扩增。已经开发了各种WGA试剂盒，例如Repli-g Mini试剂盒（Qiagen）和GenomiPhi试剂盒（GE卫生保健）。因此，选择适合我们研究的理想套件非常重要。在这项研究中，在比较了三种WGA试剂盒之后，差异表明从大肠杆菌基因组DNA扩增的WGA产物代表了不同的扩增效率。

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  • 来源
  • 会议地点 Beijing(CN);Beijing(CN)
  • 作者

    Ma Chao; Li Zhiyang; Li Xiaolong; He Lei; He Nongyue;

  • 作者单位

    State Key Laboratory of Bioelectronics,School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China Department of Chemistry and Life Science, Maoming University, Maoming 525000, China;

    State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China;

    State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China;

    State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China;

    State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Whole Genome Amplification (WGA); Amplification efficiency; Escherichia coli;

    机译：全基因组扩增（WGA）；扩增效率；大肠杆菌;

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