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首页> 外文会议>7th China-Japan International Congress of Microbiology Shanghai Symposium-2000 4-6 August 2000 Shanghai >Methicillin-resistant staphylococci hvr genotypes and their relationship to the resistance
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Methicillin-resistant staphylococci hvr genotypes and their relationship to the resistance

机译：耐甲氧西林金黄色葡萄球菌基因型及其与耐药性的关系

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this study was conducted to gain the HVR-PCR genotype of methicillin-resistant Staphylococci in local hospitals and compare it with its antibiograms, hoping it helpful for selecting effective antibacterial agents, moreover, to discuss preliminarily its role in molecular epidemiology. The minimal inhibitioy concentrations(MICs)of 86 MRSA, 10 5MSSE, 8MRSHand 5MSSH clinical isolates which come from 4 local hospitals were tested by serial two-fold agar dilution method, their DNA were extracted by moved basiclytic method, whose polymerase chain reaction (PCR) products amplified based on the size of mec-associated hypervarriable region(HVR)were analyzed by PAG vertical and agarose gel electrophoresis. The result shows that MRSA, MRSE and MRSH were grouped into 4,3 and 2 HVR genotypes, respectively, based on the size of the PCR products. The PCR products amplified from 9 of 10 MRSE isolates were the same as products amplified from MRSA isolates. MRSA strains in this study were mainly HVR genotypes A and D, which accounted for 52.32
机译：这项研究的目的是在当地医院获得耐甲氧西林葡萄球菌的HVR-PCR基因型，并将其与抗菌素谱图进行比较，希望有助于选择有效的抗菌剂，并初步探讨其在分子流行病学中的作用。采用连续两倍琼脂稀释法检测了来自4家当地医院的86种MRSA，10种5MSSE，8MRSHand 5MSSH临床分离株的最低抑菌浓度（MICs），采用移动碱性水解法提取了其DNA，并进行了聚合酶链反应（PCR）。 PAG垂直和琼脂糖凝胶电泳分析了基于mec相关高变区（HVR）大小扩增的产物。结果表明，根据PCR产物的大小，MRSA，MRSE和MRSH分别分为4、3和2个HVR基因型。从10个MRSE分离物中的9个扩增的PCR产物与从MRSA分离物中扩增的产物相同。本研究中的MRSA菌株主要为HVR基因型A和D，占52.32。

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