• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文会议>The 7th international medicinal mushroom conference >The MatA Mating Type Locus of Pleurotus ostreatus
【24h】

The MatA Mating Type Locus of Pleurotus ostreatus

机译：平菇的MatA交配型基因座

获取原文
获取原文并翻译 | 示例
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The edible mushroom Pleurotus ostreatus is one of the most cultivated species worldwide.Fruiting bodies emerge on the dikaryon and this requires well-coordinated actions of the mating type genes.Pleurotus species are characterized by a tetrapolar mating incompatibility system which bases on two unlinked mating loci matA and matB with multiple alleles each.Every monokaryon has a specific mating type defined by its individual matA and matB alleles.Two monokaryons are compatible only in case of different alleles at both mating type loci.Only compatible monokaryons form a fertile dikaryon.In current research,we are elucidating the structure of different alleles of the matA locus.The locus contains genes for two distinct types of homeodomain transcription factors.We are cloning the genes from strains of different A mating type activity.In the future,we want to transform the genes into suitable monokaryotic hosts for functional analyses.As a first morphological marker,we will observe clamp cell formation at hyphal septa of resulting transformants.To study effects of transformed matA genes on later steps of development such as initiation of mushroom production and meiotic basidiospore formation,transformants might be appointed in classical genetic crosses and progeny analyses.To this end,reliable conditions for fruiting of dikaryotic strains in the laboratory are required.We therefore describe here also successful experiments of mushroom production under laboratory conditions.
机译：食用菌平菇是世界上栽培最多的物种之一，在双核体上出现了果体，这需要交配型基因的良好协调作用。平菇物种的特征是基于两个未连接的交配位点的四极交配不相容系统。 matA和matB各自具有多个等位基因。每个单体核均具有由其单独的matA和matB等位基因定义的特定交配类型。两个单体核仅在两个交配类型基因座处的等位基因不同的情况下才相容，只有相容的单体核形成可育的双核子。通过研究，我们正在阐明matA基因座的不同等位基因的结构。该基因座包含两种不同类型的同源域转录因子的基因。我们正在从具有不同A交配类型活性的菌株中克隆该基因。将来，我们希望转化将基因放入合适的单核宿主中进行功能分析。作为第一个形态学标记，我们将观察钳c为了研究转化的matA基因对蘑菇发育的初始阶段和减数分裂的担子孢子形成等后续发育步骤的影响，可以在经典的遗传杂交和后代分析中指定转化子。为此，需要可靠的条件因此需要在实验室条件下成功地进行蘑菇生产实验。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号