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Mechanism Regulating HvAACT1 Expression in Barley

机译:大麦中HvAACT1表达的调控机制

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摘要

Recently, a gene responsible for Al-activated citrate secretion (HvAACT1) has been identified in barley. In the present study, to examine the mechanism regulating the expression of HvAACT1, we compared the sequence of UTR regions between Al-sensitive and Al-tolerant cultivars. In the 3'-UTR region there was no correlation between Al tolerance and the sequence difference. In the 5'-region, we found that a specific region was inserted in the ORF upstream only in the Al-tolerant cultivars. 5'-RACE analysis showed that in Al-tolerant cultivars, there were multiple transcription start sites. These results suggest that this region may be involved in the higher expression of HvAACT1.
机译:最近,已在大麦中鉴定出负责Al激活的柠檬酸盐分泌(HvAACT1)的基因。在本研究中,为了检查调节HvAACT1表达的机制,我们比较了铝敏感品种和耐铝品种之间的UTR区序列。在3'-UTR区域中,A1耐性和序列差异之间没有相关性。在5'区域,我们发现仅在耐铝品种中在ORF上游插入了特定区域。 5'-RACE分析表明,在耐铝品种中,有多个转录起始位点。这些结果表明该区域可能参与HvAACT1的更高表达。

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