Artificial Golgi Apparatus: Direct Monitoring of Glycosylation Reactions on Automated Glycosynthesizer

机译：人造高尔基仪：自动化糖合成仪上糖基化反应的直接监测

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Enzymatic synthesis is potential method for the construction of glycoconjugates as well as conventional chemical synthesis. Use of glycosyltransferases in the synthetic schemes has accelerated practical synthesis of oligosaccharides and related compounds. However, there is no systematic approach for establishing "automated glycosynthesizer" based on the combined chemical and enzymatic strategy. We have found that water-soluble glycopolymers bearing multivalent sugars become excellent glycosyl acceptor substrates of glycosyltransferases. Affinity of enzymes with multivalent glycosyl acceptors was drastically enhanced by polymeric sugar-cluster effect. Some designed linkers that can be recognized by specific enzyme permitted release of products from polymer supports under mild and selective conditions.

机译：酶促合成是构建糖缀合物以及常规化学合成的潜在方法。在合成方案中使用糖基转移酶已经加速了寡糖和相关化合物的实际合成。但是，没有基于化学和酶结合策略建立“自动化糖合成仪”的系统方法。我们已经发现带有多价糖的水溶性糖聚合物成为糖基转移酶的优良糖基受体底物。聚合糖簇效应大大增强了酶与多价糖基受体的亲和力。某些可以被特定酶识别的设计连接子允许产物在温和和选择性的条件下从聚合物载体上释放出来。

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来源
《ACS Symposium Series 873; American Chemical Society Meeting; 2002; Boston,MA(US)》|2002年|P.113-124|共12页
会议地点 BostonMA(US)
作者
Shin-Ichiro Nishimura; Noriko Nagahori; Reiko Sadamoto; Kcnji Monde; Kenichi Niikura;
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作者单位

Laboratory for Bio-Macromolecular Chemistry, Division of Biological Sciences, Graduate School of Science, Hokkaido University, N21 W11, Kita-ku, Sapporo 001-0011, Japan;

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原文格式 PDF
正文语种 eng
中图分类生物工程学（生物技术）;
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1. Artificial Golgi Apparatus: Globular Protein-like Dendrimer Facilitates Fully Automated Enzymatic Glycan Synthesis [J] . Takahiko Matsushita, Izuru Nagashima, Masataka Fumoto, Journal of the American Chemical Society . 2010,第46期

机译：人工高尔基体设备：球状蛋白状树状大分子促进全自动酶促聚糖合成。
2. Do the Golgi Glycosylation Enzymes Cycle between the Endoplasmic Reticulum and the Golgi Apparatus? [J] . Satoh Ayano, Honjo Yasuko Trends in Glycoscience and Glycotechnology . 2017,第165a167期

机译：在内质网和高尔基装置之间进行高尔基糖基化酶循环吗？
3. Do the Golgi Glycosylation Enzymes Cycle between the Endoplasmic Reticulum and the Golgi Apparatus? [J] . Satoh Ayano, Honjo Yasuko Trends in Glycoscience and Glycotechnology . 2017,第165a167期

机译：在内质网和高尔基装置之间进行高尔基糖基化酶循环吗？
4. Artificial Golgi Apparatus: Direct Monitoring of Glycosylation Reactions on Automated Glycosynthesize [C] . Shin-Ichiro Nishimura, Noriko Nagahori, Reiko Sadamoto, American Chemical Society Meeting . 2004

机译：人造高尔基装置：直接监测糖基化反应自动化糖合成的
5. A new role and mechanistic model for the conserved oligomeric Golgi (COG) complex in tethering retrograde vesicles to the Golgi apparatus. [D] . Willett, Rose A. 2014

机译：保守的低聚高尔基体（COG）复合体在逆行囊泡与高尔基体的束缚中的新作用和机制模型。
6. Mechanisms of glycosylation and sulfation in the Golgi apparatus: evidence for nucleotide sugarucleoside monophosphate and nucleotide sulfateucleoside monophosphate antiports in the Golgi apparatus membrane. [O] . J M Capasso, C B Hirschberg 1984

机译：高尔基体中糖基化和硫酸化的机理：高尔基体膜中核苷酸糖/核苷单磷酸和核苷酸硫酸盐/核苷单磷酸的反证据。
7. Mechanisms of glycosylation and sulfation in the Golgi apparatus: evidence for nucleotide sugar/nucleoside monophosphate and nucleotide sulfate/nucleoside monophosphate antiports in the Golgi apparatus membrane. [O] . Capasso, J M, Hirschberg, C B 1984

机译：高尔基体中糖基化和硫酸化的机理：高尔基体膜中核苷酸糖/核苷单磷酸和核苷酸硫酸盐/核苷单磷酸的反证据。

Artificial Golgi Apparatus: Direct Monitoring of Glycosylation Reactions on Automated Glycosynthesizer

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