首页> 外文会议>ACS Symposium Series 892; Pan-Pacific Conference on Pesticide Science; 20030601-04; Honolulu,HI(US) >ELISA and Liquid Chromatography/Mass Spectrometry/Mass Spectrometry Methods for Sulfentrazone and Its Acid Metabolite in Groundwater Samples
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ELISA and Liquid Chromatography/Mass Spectrometry/Mass Spectrometry Methods for Sulfentrazone and Its Acid Metabolite in Groundwater Samples

机译:地下水样品中次硫zone及其酸代谢产物的酶联免疫吸附测定和液相色谱/质谱/质谱方法

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摘要

Sulfentrazone represents a new class of herbicides (aryl triazolinones). It inhibits the protoporphyrinogen oxidase (PPO) in the plant chlorophyll biosynthetic pathway. The major metabolite identified in the water is sulfentrazone-3-carboxylic acid (SCA). An ELISA (enzyme-linked immunosorbent assay) test kit has been used as an analytical screening tool for the water samples (including well, lysimeter, and other source water) from three groundwater studies. Compared to the conventional chemical assay, ELISA uses minimum chemicals (solvents and reagents) and is simpler and time- and cost-effective. LC/MS/MS (liquid chromatograph equipped with triple quadruple mass spectrometry) was used for the confirmation of all positive and partial negative residues. Method development and comparisons of precision, accuracy, cross-reactivity, recovery, and false negatives and positives for ELISA and LC/MS/MS are discussed herein.
机译:次磺zone代表一类新的除草剂(芳基三唑啉酮)。它抑制植物叶绿素生物合成途径中的原卟啉原氧化酶(PPO)。在水中鉴定出的主要代谢物是次en 3羧酸(SCA)。 ELISA(酶联免疫吸附测定)测试试剂盒已用作对来自三个地下水研究的水样(包括井,溶渗仪和其他源水)进行分析的筛选工具。与常规化学分析相比,ELISA使用的化学物质最少(溶剂和试剂),并且更简单,更省时,更经济。 LC / MS / MS(配备三重四极杆质谱仪的液相色谱仪)用于确认所有阳性和部分阴性残留物。本文讨论了方法开发以及ELISA和LC / MS / MS的精密度,准确性,交叉反应性,回收率以及假阴性和假阳性的比较。

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