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Extended Depth-of-Field Microscopy with a High-Speed Deformable Mirror

机译:具有高速可变形反射镜的扩展景深显微镜

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摘要

Wide-field fluorescence microscopy is generally limited to either small volumes or low temporal resolution. We present a microscope add-on that provides fast, light-efficient extended depth-of-field (EDOF) using a deformable mirror of update rate 20kHz. Out-of-focus contributions in the raw EDOF images are suppressed with a de-convolution algorithm derived directly from the microscope 3D optical transfer function. Demonstrations of the benefits of EDOF microscopy are shown with GCaMP-labeled mouse brain tissue.
机译:宽视场荧光显微镜通常限于小体积或低时间分辨率。我们介绍了一种显微镜附件,它使用可更新频率20kHz的可变形反射镜提供快速,高效的光扩展景深(EDOF)。原始EDOF图像中的散焦贡献可通过直接从显微镜3D光学传递函数得出的解卷积算法来抑制。用GCaMP标记的小鼠脑组织显示了EDOF显微镜的好处。

著录项

  • 来源
  • 会议地点 San Francisco(US)
  • 作者单位

    Dept. of Physics, Boston University, 590 Commonwealth Avenue, Boston, MA 02215,Photonics Center, Boston University, 8 Saint Mary's St. Boston MA, 02215;

    Dept. of Mechanical Engineering, Boston University, 110 Cummington Mall, Boston, MA 02215,Photonics Center, Boston University, 8 Saint Mary's St. Boston MA, 02215;

    Dept. of Physics, Boston University, 590 Commonwealth Avenue, Boston, MA 02215,Searle Center for Advanced Learning and Teaching, Northwestern University, 627 Dartmouth Place Evanston, IL 60208;

    Dept. of Mechanical Engineering, Boston University, 110 Cummington Mall, Boston, MA 02215,Photonics Center, Boston University, 8 Saint Mary's St. Boston MA, 02215;

    Dept. of Biomedical Engineering, Boston University, 44 Cummington Mall, Boston, MA 02215,Photonics Center, Boston University, 8 Saint Mary's St. Boston MA, 02215;

  • 会议组织
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Fluorescence Microscopy; Adaptive Optics; Deconvolution; Extended Depth of Field; Brain Imaging; Neuron Imaging;

    机译:荧光显微镜自适应光学去卷积景深扩展脑成像;神经元成像;

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