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New sensorless wavefront estimation approach for two photon scanning microscopy

机译:用于两个光子扫描显微镜的新的无传感器波前估计方法

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摘要

The landscape of biomedical research in neuroscience has changed dramatically in recent years as a result of spectacular progress in dynamic microscopy. In this context Adaptive Optics allows in-depth imaging by correcting aberrations induced by the biological sample, the key issue being then the ability to perform an accurate and reliable wavefront sensing (WFS). We present here a limitation of modal sensorless WFS in the case of a heterogeneous medium. We then build a new method called Axially-Locked Modal Sensorless (ALMS) that exploits these heterogeneities to overcome this limitation. The new method is simulated and compared to standard modal sensorless. The simulation results show a more accurate wavefront estimation even in the case of a strongly aberrated biological media.
机译:由于动态显微镜技术取得了令人瞩目的进步,近年来神经科学领域的生物医学研究领域发生了巨大变化。在这种情况下,自适应光学器件可以通过校正生物样品引起的像差来进行深度成像,然后,关键问题是执行准确而可靠的波前感测(WFS)的能力。在异构介质的情况下,我们在这里提出了模式无传感器WFS的局限性。然后,我们建立了一种称为轴向锁定模态无传感器(ALMS)的新方法,该方法利用这些异质性来克服此限制。对新方法进行了仿真,并将其与标准模式无传感器相比较。仿真结果显示,即使在严重畸变的生物介质中,波前估计也更加准确。

著录项

  • 来源
  • 会议地点 San Francisco(US)
  • 作者单位

    ONERA - the French Aerospace Lab, F-92320 Chatillon, France;

    Institut Fresnel, UMR 7249 CNRS, Aix-Marseille Universite, F-13013 Marseille, France,INMED, INSERM U901, Aix-Marseille Universite, F-13273 Marseille, France;

    ONERA - the French Aerospace Lab, F-92320 Chatillon, France;

    ONERA - the French Aerospace Lab, F-92320 Chatillon, France;

    ONERA - the French Aerospace Lab, F-92320 Chatillon, France;

    Institut Fresnel, UMR 7249 CNRS, Aix-Marseille Universite, F-13013 Marseille, France;

    INMED, INSERM U901, Aix-Marseille Universite, F-13273 Marseille, France;

    INMED, INSERM U901, Aix-Marseille Universite, F-13273 Marseille, France;

    INMED, INSERM U901, Aix-Marseille Universite, F-13273 Marseille, France;

    Institut Fresnel, UMR 7249 CNRS, Aix-Marseille Universite, F-13013 Marseille, France;

    Institut Fresnel, UMR 7249 CNRS, Aix-Marseille Universite, F-13013 Marseille, France;

    Institut Fresnel, UMR 7249 CNRS, Aix-Marseille Universite, F-13013 Marseille, France,INMED, INSERM U901, Aix-Marseille Universite, F-13273 Marseille, France;

  • 会议组织
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    adaptive optics; microscopy; multi-photon; modal sensorless; axially-locked; sample dependence; neurobiology;

    机译:自适应光学显微镜多光子模态无传感器轴向锁定样本依赖性神经生物学;

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