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首页> 外文会议>Advanced Biomedical and Clinical Diagnostic and Surgical Guidance Systems XIV >Raman spectroscopy and immunohistochemistry for schwannoma characterization: a case study
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Raman spectroscopy and immunohistochemistry for schwannoma characterization: a case study

机译：拉曼光谱和免疫组织化学表征神经鞘瘤的案例研究

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摘要

The schwannomas is a tumour of the tissue that covers nerves, called the nerve sheath. Schwannomas are often benign tumors of the Schwan cells, which are the principal glia of the peripheral nervous system (PNS). Preoperative diagnosis of this lesion usually is difficult, therefore, new techniques are being studied as pre surgical evaluation. Among these, Raman spectroscopy, that enables the biochemical identification of the tissue analyzed by their optical properties, may be used as a tool for schwannomas diagnosis. The aim of this study was to discriminate between normal nervous tissue and schwannoma through the confocal Raman spectroscopy and Raman optical fiber-based techniques combined with immunohistochemical analysis. Twenty spectra were analyzed from a normal nerve tissue sample (10) and schwannoma (10) by Holospec f / 1.8 (Kayser Optical Systems) coupled to an optical fiber with a 785nm laser line source. The data were pre-processed and vector normalized. The average analysis and standard deviation was performed associated with cluster analysis. AML, 1A4, CD34, Desmin and S-100 protein markers were used for immunohistochemical analysis. Immunohistochemical analysis was positive only for protein S-100 marker which confirmed the neural schwanomma originality. The immunohistochemistry analysis were important to determine the source of the injury, whereas Raman spectroscopy were able to differentiated tissues types indicating important biochemical changes between normal and benign neoplasia.
机译：神经鞘瘤是一种覆盖神经的组织肿瘤，称为神经鞘。 Schwannomas通常是Schwan细胞的良性肿瘤，它是周围神经系统（PNS）的主要神经胶质。通常很难对这种病变进行术前诊断，因此，正在研究新技术作为术前评估。其中，拉曼光谱法可以通过光学特性对组织进行生化鉴定，可以用作神经鞘瘤诊断的工具。这项研究的目的是通过共聚焦拉曼光谱法和基于拉曼光纤的技术结合免疫组织化学分析来区分正常神经组织和神经鞘瘤。通过Holospec f / 1.8（Kayser Optical Systems），通过耦合至785nm激光线光源的光纤，从正常神经组织样本（10个）和神经鞘瘤（10个）中分析了二十个光谱。数据经过预处理和向量归一化。进行均值分析和标准差与聚类分析。 AML，1A4，CD34，Desmin和S-100蛋白标志物用于免疫组化分析。免疫组织化学分析仅对蛋白S-100标记呈阳性，这证实了神经性神经鞘瘤的独创性。免疫组织化学分析对于确定损伤的来源很重要，而拉曼光谱法能够区分组织类型，表明正常和良性瘤形成之间重要的生化变化。

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  • 来源
  • 会议地点 San Francisco CA(US)
  • 作者

    Lazaro Pinto Medeiros Neto; Maurilio Jose das Chagas; Luis Felipe CS Carvalho; Isabelle Ferreira; Laurita dos Santos; Marcelo Haddad; Vinicius Loddi; Airton Abrahao Martin;

  • 作者单位

    UNIVAP, Instituto de Pesquisa e Desenvolvimento, Laboratorio de Espectroscopia Vibracional Biomedica, Avenida Shishima Hifumi, 2911, Sao Jose dos Campos, Sao Paulo - SP, Brasil - CEP: 12.244-000;

    UNIVAP, Instituto de Pesquisa e Desenvolvimento, Laboratorio de Espectroscopia Vibracional Biomedica, Avenida Shishima Hifumi, 2911, Sao Jose dos Campos, Sao Paulo - SP, Brasil - CEP: 12.244-000;

    UNIVAP, Instituto de Pesquisa e Desenvolvimento, Laboratorio de Espectroscopia Vibracional Biomedica, Avenida Shishima Hifumi, 2911, Sao Jose dos Campos, Sao Paulo - SP, Brasil - CEP: 12.244-000;

    UNIVAP, Instituto de Pesquisa e Desenvolvimento, Laboratorio de Espectroscopia Vibracional Biomedica, Avenida Shishima Hifumi, 2911, Sao Jose dos Campos, Sao Paulo - SP, Brasil - CEP: 12.244-000;

    UNIVAP, Instituto de Pesquisa e Desenvolvimento, Laboratorio de Espectroscopia Vibracional Biomedica, Avenida Shishima Hifumi, 2911, Sao Jose dos Campos, Sao Paulo - SP, Brasil - CEP: 12.244-000;

    UNIVAP, Instituto de Pesquisa e Desenvolvimento, Laboratorio de Espectroscopia Vibracional Biomedica, Avenida Shishima Hifumi, 2911, Sao Jose dos Campos, Sao Paulo - SP, Brasil - CEP: 12.244-000;

    UNIVAP, Instituto de Pesquisa e Desenvolvimento, Laboratorio de Espectroscopia Vibracional Biomedica, Avenida Shishima Hifumi, 2911, Sao Jose dos Campos, Sao Paulo - SP, Brasil - CEP: 12.244-000;

    UNIVAP, Instituto de Pesquisa e Desenvolvimento, Laboratorio de Espectroscopia Vibracional Biomedica, Avenida Shishima Hifumi, 2911, Sao Jose dos Campos, Sao Paulo - SP, Brasil - CEP: 12.244-000;

  • 会议组织
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Raman spectroscopy; Optical spectroscopy; Schwannoma; Immunohistochemistry; nerve sheath;

    机译：拉曼光谱光谱学； Schwannoma；免疫组织化学；神经鞘;

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