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首页> 外文会议>Advanced Biomedical and Clinical Diagnostic and Surgical Guidance Systems XV >DNA detection and single nucleotide mutation identification using SERS for molecular diagnostics and global health
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DNA detection and single nucleotide mutation identification using SERS for molecular diagnostics and global health

机译：使用SERS进行DNA检测和单核苷酸突变鉴定以进行分子诊断和全球健康

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摘要

Nucleic acid-based molecular diagnostics at the point-of-care (POC) and in resource-limited settings is still a challenge. We present a sensitive yet simple DNA detection method with single nucleotide polymorphism (SNP) identification capability. The detection scheme involves sandwich hybridization of magnetic beads conjugated with capture probes, target sequences, and ultrabright surface-enhanced Raman Scattering (SERS) nanorattles conjugated with reporter probes. Upon hybridization, the sandwich probes are concentrated at the detection focus controlled by a magnetic system for SERS measurements. The ultrabright SERS nanorattles, consisting of a core and a shell with resonance Raman reporters loaded in the gap space between the core and the shell, serve as SERS tags for ultrasensitive signal detection. Specific DNA sequences of the malaria parasite Plasmodium falciparum and dengue virus 1 (DENV1) were used as the model marker system. Detection limit of approximately 100 attomoles was achieved. Single nucleotide polymorphism (SNP) discrimination of wild type malaria DNA and mutant malaria DNA, which confers resistance to artemisinin drugs, was also demonstrated. The results demonstrate the molecular diagnostic potential of the nanorattle-based method to both detect and genotype infectious pathogens. The method's simplicity makes it a suitable candidate for molecular diagnosis at the POC and in resource-limited settings.
机译：在护理点（POC）和资源有限的环境中，基于核酸的分子诊断仍然是一个挑战。我们提出了一种具有单核苷酸多态性（SNP）识别能力的敏感而简单的DNA检测方法。该检测方案涉及与捕获探针，靶标序列缀合的磁珠和与报告子探针缀合的超亮表面增强拉曼散射（SERS）纳米ra的夹心杂交。杂交后，夹心探针集中在由磁性系统控制的检测焦点上，以进行SERS测量。超亮的SERS纳米ttle，由核和壳组成，共振拉曼报告分子装载在核和壳之间的间隙中，用作超敏感信号检测的SERS标签。疟疾寄生虫恶性疟原虫和登革热病毒1（DENV1）的特定DNA序列用作模型标记系统。检出限约为100个。还证明了野生型疟疾DNA和突变型疟疾DNA的单核苷酸多态性（SNP）区分，赋予了对青蒿素药物的抗性。结果证明了基于纳米拨浪鼓的方法的分子诊断潜力，可同时检测和区分感染性病原体。该方法的简便性使其成为在POC和资源有限的环境中进行分子诊断的合适候选者。

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