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首页> 外文会议>Advanced Biomedical and Clinical Diagnostic Systems III; Progress in Biomedical Optics and Imaging; vol.6 no.7 >Biosensor-Controlled Gene Therapy/Drug Delivery with Nanoparticles for Nanomedicine
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Biosensor-Controlled Gene Therapy/Drug Delivery with Nanoparticles for Nanomedicine

机译：生物传感器控制的基因治疗/纳米药物与纳米药物的药物输送。

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Nanomedicine involves cell-by-cell regenerative medicine, either repairing cells one at a time or triggering apoptotic pathways in cells that are not repairable. Multilayered nanoparticle systems are being constructed for the targeted delivery of gene therapy to single cells. Cleavable shells containing targeting, biosensing, and gene therapeutic molecules are being constructed to direct nanoparticles to desired intracellular targets. Therapeutic gene sequences are controlled by biosensor-activated control switches to provide the proper amount of gene therapy on a single cell basis. The central idea is to set up gene therapy "nanofactories" inside single living cells. Molecular biosensors linked to these genes control their expression. Gene delivery is started in response to a biosensor detected problem; gene delivery is halted when the cell response indicates that more gene therapy is not needed. Cell targeting of nanoparticles, both nanocrystals and nanocapsules, has been tested by a combination of fluorescent tracking dyes, fluorescence microscopy and flow cytometry. Intracellular targeting has been tested by confocal microscopy. Successful gene delivery has been visualized by use of GFP reporter sequences. DNA tethering techniques were used to increase the level of expression of these genes. Integrated nanomedical systems are being designed, constructed, and tested in-vitro, ex-vivo, and in small animals. While still in its infancy, nanomedicine represents a paradigm shift in thinking - from destruction of injured cells by surgery, radiation, chemotherapy to cell-by-cell repair within an organ and destruction of non-repairable cells by natural apoptosis.
机译：纳米医学涉及逐个细胞的再生医学，要么一次修复一个细胞，要么触发不可修复细胞的凋亡途径。正在构建多层纳米颗粒系统，用于将基因治疗靶向递送至单细胞。正在构建包含靶向，生物传感和基因治疗分子的可裂解壳，以将纳米颗粒导向所需的细胞内靶标。治疗性基因序列由生物传感器激活的控制开关控制，以在单个细胞的基础上提供适当量的基因治疗。中心思想是在单个活细胞内建立基因疗法“纳米工厂”。与这些基因相关的分子生物传感器控制着它们的表达。响应于生物传感器检测到的问题开始基因传递；当细胞反应表明不需要更多的基因治疗时，基因传递停止。已经通过荧光跟踪染料，荧光显微镜和流式细胞术的组合测试了纳米颗粒（纳米晶体和纳米胶囊）的细胞靶向性。共聚焦显微镜已经测试了细胞内靶向。通过使用GFP报告基因序列已经成功地观察到了基因的传递。 DNA束缚技术被用来增加这些基因的表达水平。集成的纳米医学系统正在体外，离体和小型动物中进行设计，构建和测试。纳米医学虽然还处于起步阶段，但它代表着思维方式的转变-从通过手术，放射，化学疗法破坏受损细胞到在器官内进行逐细胞修复以及通过自然凋亡破坏不可修复的细胞。

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