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首页> 外文会议>Advanced Biomedical and Clinical Diagnostic Systems III; Progress in Biomedical Optics and Imaging; vol.6 no.7 >Endoscopic ultrahigh-resolution OCT for in vivo imaging colon disease model mice
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Endoscopic ultrahigh-resolution OCT for in vivo imaging colon disease model mice

机译：内窥镜超高分辨率OCT在体内成像结肠疾病模型小鼠

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摘要

Mouse models are increasingly important for studying human GI pathology. OCT provides minimally invasive, cross-sectional images that indicate the thickness and scattering density of underlying tissue. We have developed endoscopic ultrahigh resolution OCT (UHR-OCT) for the purpose of in vivo imaging in mouse colon. The reduced scale of the mouse colon makes tissue light penetration much less problematic, and high resolution acutely necessary. Higher lateral resolution requires a departure from the traditional cemented GRIN lens design. We support the need for better chromatic aberration than can be achieved by a GRIN lens using commercial raytracing software. We have designed and built a 2mm diameter endoscopic UHR-OCT system achromatized for 770-1020nm for use with a Titanium:sapphire laser with 260 nm bandwidth at full-width-half-maximum centered at 800 nm while achieving a 4.4um lateral spot dimension at focus. A pair of KZFSN5/SFPL53 doublets provides excellent primary and secondary color correction to maintain wide bandwidth through the imaging depth. A slight deviation from normal beam exit angle suppresses collection of the strong back reflection at the exit window surface. The novel design endoscope was built and characterized for through focus bandwidth, axial resolution, signal to noise, and lateral spot dimension. Performance is demonstrated on a variety of ex vivo tissues and in situ mouse colon. Ultrahigh-resolution images of mouse tissue enable the visualization of microscopic features, including crypts that have previously been observed with standard resolution OCT in humans but were too small to see in mouse tissue. Resolution near the cellular level is potentially capable of identifying abnormal crypt formation and dysplastic cellular organization.
机译：鼠标模型对于研究人类GI病理学越来越重要。 OCT提供了微创的横截面图像，可显示基础组织的厚度和散射密度。我们已经开发出内窥镜超高分辨率OCT（UHR-OCT），用于在小鼠结肠中进行体内成像。小鼠结肠的缩小尺寸使组织光穿透的问题减少了很多，并且迫切需要高分辨率。更高的横向分辨率需要脱离传统的胶合GRIN透镜设计。我们支持需要比使用商业射线追踪软件的GRIN透镜更好的色差的需求。我们设计并制造了一个直径为2mm的内窥镜UHR-OCT系统，其消色差范围为770-1020nm，可与260nm带宽的钛蓝宝石激光器一起使用，其最大宽度为一半，最大集中于800nm，同时实现4.4um的横向光斑重点关注。一对KZFSN5 / SFPL53双重透镜可提供出色的原色和副色校正，以在整个成像深度上保持较宽的带宽。与法线光束出射角的微小偏差会抑制在出射窗表面聚集强烈的反光。这种新型设计的内窥镜是通过聚焦带宽，轴向分辨率，信噪比和横向光斑尺寸来构建和表征的。在各种离体组织和原位小鼠结肠上都证明了其性能。小鼠组织的超高分辨率图像可实现微观特征的可视化，包括以前在人类中以标准分辨率OCT观察到的隐窝，但这些隐窝太小而无法在小鼠组织中看到。接近细胞水平的分辨率可能能够识别异常的隐窝形成和发育异常的细胞组织。

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