• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文会议>Advanced Biomedical and Clinical Diagnostic Systems IV; Progress in Biomedical Optics and Imaging; vol.7 no.3 >Diffuse spectroscopy for inhomogeneous metal nanoparticle assays
【24h】

Diffuse spectroscopy for inhomogeneous metal nanoparticle assays

机译：扩散光谱法用于非均质金属纳米颗粒测定

获取原文
获取原文并翻译 | 示例
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

We describe a compact computational spectroscopy platform optimized for molecular recognition using metal nanoparticle assays. The objective is motivated by the urgent need for low-cost, portable and high-throughput sensors for point-of-care (POC) clinical diagnostics. Nanoparticle based sensing has been successfully demonstrated for diagnosis and monitoring of infectious diseases, drug discovery, proteomics, and biological agent detection. Molecular binding on the nanoparticle surface is transuded into an optical signal by modification of the nanoparticle extinction spectrum (via a shift in Localized Surface Plasmon Resonance) or by modification of the molecular scattering spectrum (via Surface Enhanced Raman Scattering). Translating a nanoparticle -based molecular recognition system into a functional miniature hand-held biosensor requires spectrometer designs optimized to large area nanoparticle assays and integrated spectral filtering to improve the signal specificity. Large population sampling with small population sensitivity is essential to highly sensitive nanoparticle assay analysis. We describe a multimodal multiplex spectroscopy (MMS) platform that samples the spectral response of up to 10~6 populations of 10-100 nanoparticles in parallel. The advantages of MMS approach include: extremely high signal throughput due to its large aperture and high resolution with small form factor. We will demonstrate a nanoparticle biosensor platform based on MMS. Ultimately, a fully integrated functional miniature nanoparticle based biosensor for real time disease diagnosis in whole blood assays can be realized.
机译：我们描述了一种紧凑的计算光谱平台，该平台针对使用金属纳米颗粒测定的分子识别进行了优化。迫切需要低成本，便携式和高吞吐量的即时诊断（POC）临床诊断传感器，从而推动了这一目标。基于纳米粒子的传感已成功证明可用于传染病的诊断和监测，药物发现，蛋白质组学和生物制剂检测。通过改变纳米粒子的消光光谱（通过局部表面等离振子共振）或通过改变分子散射光谱（通过表面增强拉曼散射），将纳米粒子表面上的分子结合转化为光信号。将基于纳米颗粒的分子识别系统转化为功能的微型手持式生物传感器，需要针对大面积纳米颗粒测定进行优化的光谱仪设计，并需要集成光谱过滤以提高信号特异性。高种群抽样和低种群敏感性对于高度敏感的纳米颗粒分析至关重要。我们描述了一种多峰多重光谱学（MMS）平台，该平台可并行采样多达10〜6个10-100个纳米颗粒的光谱响应。 MMS方法的优点包括：由于其大孔径和小尺寸的高分辨率，因此具有极高的信号吞吐量。我们将演示基于MMS的纳米颗粒生物传感器平台。最终，可以实现用于全血测定中实时疾病诊断的完全集成的基于功能微型纳米颗粒的生物传感器。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号