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Evanescent imaging ellipsometry based microarray readers

机译：基于逝成像椭偏仪的微阵列读取器

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Microarrays are being widely used in genomic, proteomic, and diagnostic applications. The binding events to the microarrays are measured with fluorescent labels. Fluorescent microarray readers offer high sensitivity and normalization of the reference and test samples. The use of labels increases the number of steps involved in array testing, concerns about storage labels, and cost of additional labeling steps. This paper describes an alternative approach that does not require the use of fluorescent or other labels. The binding events on the microarray introduce changes in polarization of the illuminated light which is measured to determine the concentrations of biomolecules bound to the microarray. Oligonucleotide microarrays were synthesized and tested on the imaging microarray reader. The refractive index changes of 0.006 and changes in thickness of 1 nm are demonstrated at a spatial resolution of 20 μm over a field of view of 1 cm~2. This ellipsometric technique offers an attractive alternative to fluorescence-based measurement and could be very valuable in some of the genomic, proteomic, diagnostic, and sensing applications.
机译：微阵列被广泛用于基因组，蛋白质组和诊断应用中。用荧光标记物测量与微阵列的结合事件。荧光微阵列读取器可提供高灵敏度，并能对参考样品和测试样品进行标准化。标签的使用增加了阵列测试中涉及的步骤数量，对存储标签的担忧以及附加标签步骤的成本。本文介绍了一种不需要使用荧光标记或其他标记的替代方法。微阵列上的结合事件引起照明光的偏振变化，该变化被测量以确定结合至微阵列的生物分子的浓度。合成寡核苷酸微阵列并在成像微阵列阅读器上进行测试。在1 cm〜2的视场中，在20μm的空间分辨率下显示出0.006的折射率变化和1 nm的厚度变化。这种椭圆仪技术可以替代基于荧光的测量方法，并且在某些基因组，蛋白质组学，诊断和传感应用中可能非常有价值。

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