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Miniature Biochip System for Bioenvironmental Applications

机译：微型生物芯片系统，用于生物环境

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摘要

An integrated multi-functional biochip based on integrated circuit complementary metal oxide semiconductor (CMOS) sensor array for use in medical diagnostics and pathogen detection has been described. The usefulness and potential of the biochip as a rapid, inexpensive screening tool for detection of bioenvironmental pathogens will be demonstrated. Detection of aerosolized spores was achieved by coupling the miniature system to a portable bioaerosol sampler, and the performance of the antibody-based recognition and enzyme amplification method was evaluated. The bioassay performance was found to be compatible with the air sampling device, and the enzymatic amplification was found to be an attractive amplification method for detection of low spore concentrations. The combined portable bioaerosol sampler and miniature biochip system detected 100 B. globigii spores, corresponding to 17 aerosolized spores/L of air.
机译：已经描述了用于医疗诊断和病原体检测的基于集成电路互补金属氧化物半导体（CMOS）传感器阵列的集成多功能生物芯片。将证明生物芯片作为用于检测生物环境病原体的快速，廉价的筛选工具的有用性和潜力。雾化孢子的检测是通过将微型系统与便携式生物气溶胶采样器耦合实现的，并评估了基于抗体的识别和酶扩增方法的性能。发现生物测定性能与空气采样装置兼容，并且发现酶促扩增是用于检测低孢子浓度的有吸引力的扩增方法。组合的便携式生物气溶胶采样器和微型生物芯片系统检测到100球孢杆菌孢子，相当于每升空气中有17个气雾孢子。

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