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Dynamic differential image contrast by digital holography for imaging and quantitative phase microscopy

机译:数字全息动态差分图像对比度,用于成像和定量相显微镜

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Numerical managing and manipulation of complex wavefronts is performed to retrieve Differential Image Contrast (DIC) visualization starting from Digital Holographic (DH) recording. Dynamical Differential Holographic Image Contrast (DDHIC) is the name of the proposed technique. It dispenses from special optics and/or complex setup configurations with moveable components, as usually occurs in classical DIC. DDHIC presents as an appropriate method for investigating objects experiencing dynamic evolution during the measurement. In fact, it will be proved that it's useful for floating samples since it allows, from a single recording, to set a-posteriori the best conditions for DIC imaging. Dynamic representation of phase-contrast along all directions will be presented improving the specimen visualization. Furthermore, it is useful for making the proper choice of DIC key parameters as the amount of shear and the bias, with the aim to optimize the visualized phase-contrast imaging. Investigation is carried out on different biological samples
机译:从数字全息(DH)记录开始,执行复杂波前的数值管理和操作以检索差分图像对比度(DIC)可视化。动态差分全息图像对比度(DDHIC)是所提出技术的名称。像传统的DIC中通常会发生的那样,它无需使用特殊的光学器件和/或带有可移动组件的复杂设置配置。 DDHIC提出了一种适当的方法来调查在测量过程中经历动态演变的对象。实际上,将证明它对浮动样本很有用,因为它允许从单次记录设置后验DIC成像的最佳条件。将显示沿所有方向的相衬的动态表示,以改善标本的可视化效果。此外,它对于正确选择DIC关键参数(如剪切量和偏置量)很有用,目的是优化可视化的相衬成像。对不同的生物样本进行调查

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