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首页> 外文会议>Advanced microscopy techniques >The Zebrafish Digital Embryo: In Toto Reconstruction of Zebrafish Early Embryonic Development with Digital Scanned Laser Light Sheet Fluorescence Microscopy
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The Zebrafish Digital Embryo: In Toto Reconstruction of Zebrafish Early Embryonic Development with Digital Scanned Laser Light Sheet Fluorescence Microscopy

机译：斑马鱼数字胚胎：数字扫描激光灯片荧光显微镜在斑马鱼早期胚胎发育的总重建中。

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摘要

The analysis of all cell movements and all cell interactions in a vertebrate during the entire period of embryonic development is a fundamental goal in biology. Using DSLM, we recorded the development of entire zebrafish embryos in vivo and with sub-cellular resolution. By imaging at a speed of 1.5 billion volume elements per minute, image data in the order of several terabytes were acquired for each embryo over the time course of an entire day, I.e. up to a stage, in which the embryo comprises 20,000 cells and major organs are in a functional state. By using automated image processing algorithms the image data of each embryo were converted into a digital representation of the embryo (the "digital embryo"), I.e. a database with comprehensive information about migratory tracks and divisions of the embryo's cells. The digital embryos permit to follow single cells as a function of time such that the "fate" as well as the origin of the cells can be reconstructed. By means of these analyses, developmental blueprints of tissues and organs can be determined in a whole-embryo context. Defects in embryonic development or disease models can now be analyzed and understood on a quantitative level.
机译：在整个胚胎发育过程中，对脊椎动物中所有细胞运动和所有细胞相互作用的分析是生物学的基本目标。使用DSLM，我们以体内和亚细胞分辨率记录了整个斑马鱼胚胎的发育。通过以每分钟15亿个体积元素的速度成像，在一整天的时间过程中，每个胚胎获得了数TB的图像数据。直至一个阶段，在这个阶段中，胚胎包含20,000个细胞，主要器官处于功能状态。通过使用自动图像处理算法，将每个胚胎的图像数据转换为胚胎的数字表示（“数字胚胎”），即数据库，其中包含有关胚胎细胞迁移轨迹和分裂的全面信息。数字胚胎允许随时间跟随单个细胞，从而可以重建“命运”以及细胞的起源。通过这些分析，可以在整个胚胎的环境中确定组织和器官的发育蓝图。现在可以定量地分析和理解胚胎发育或疾病模型中的缺陷。

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  • 来源
    《Advanced microscopy techniques》|2009年|P.73670G.1-73670G.10|共10页
  • 会议地点 Munich(DE)
  • 作者

    Philipp J. Keller; Annette D. Schmidt; Joachim Wittbrodt; Ernst H.K. Stelzer;

  • 作者单位

    Cell Biology and Biophysics Unit, European Molecular Biology Laboratory (EMBL), Meyerhofstrasse 1, D-69117 Heidelberg, Germany Developmental Biology Unit, EMBL, Meyerhofstrasse 1, D-69117 Heidelberg, Germany;

    Developmental Biology Unit, EMBL, Meyerhofstrasse 1, D-69117 Heidelberg, Germany;

    Cell Biology and Biophysics Unit, European Molecular Biology Laboratory (EMBL), Meyerhofstrasse 1, D-69117 Heidelberg, Germany Developmental Biology Unit, EMBL, Meyerhofstrasse 1, D-69117 Heidelberg, Germany Institute of Zoology, Department for Developmental Physiology, University of Heidelberg, INF 230, D-69120 Heidelberg, Germany Institute of Toxicology and Genetics, Karlsruhe Institute of Technology (KIT), Post Office Box 3640, D-76021 Karlsruhe, Germany;

    Cell Biology and Biophysics Unit, European Mole;

  • 会议组织
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 医用物理学;
  • 关键词

    vertebrate development; automated image processing; lineage tree; light sheet-based microscopy; DSLM;

    机译：脊椎动物发育；自动图像处理；血统树基于光片的显微镜；数码相机;

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