首页> 外文会议>Advanced microscopy techniques >A comparison between coherent and spontaneous Raman scattering for biological imaging
【24h】

A comparison between coherent and spontaneous Raman scattering for biological imaging

机译:相干和自发拉曼散射在生物成像中的比较

获取原文
获取原文并翻译 | 示例

摘要

We compare imaging using coherent and spontaneous Raman scattering under biological imaging conditions. We perform spectral domain imaging of polystyrene beads and find comparable signal levels for both methods at excitation powers and concentrations most relevant for biological samples. The critical power at which the two methods provide equivalent signal levels is found to be ~1.3 mW in 10 M polystyrene beads and ~7 mW in 13 M 2-propanol. The low sample concentrations and low excitation power necessary for most biological imaging applications reduce the relative advantages offered by coherent Raman methods.
机译:我们比较在生物成像条件下使用相干和自发拉曼散射成像。我们对聚苯乙烯珠进行光谱域成像,并在与生物样品最相关的激发功率和浓度下找到两种方法的可比信号水平。发现这两种方法提供等效信号电平的临界功率在10 M聚苯乙烯珠中约为1.3 mW,在13 M 2-丙醇中约为7 mW。大多数生物成像应用所需的低样品浓度和低激发功率降低了相干拉曼方法提供的相对优势。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号